Download Recombinant DNA Exam: Zoology 452/652 - Prof. Soichi Tanda and more Exams Molecular biology in PDF only on Docsity! Zool 452/652 Recombinant DNA | ^^^f Name: Final exam (100 points) December 19, 1997 ^L^J> I Ut4? IDJi NOTE!!!!! Just use provided spaces for your answers!!!! Ql: Proto-oncogenes and anti-oncogenes are involved in tumorigenesis. Mutations in these genes often lead to tumor development. In general, dominant (or gain-of -function) mutations of proto-oncogenes are usually problems. Contrary, recessive (or loss-of -function) mutations of anti-oncogenes eventually result in development of cancer in many cases. What is likely to happen if you overexpress fa) wild type proto-oncogenes and (bl wild type anti-oncogenes in culture cells? (a) wild type proto-oncogenes (4): (b) wild type anti-oncogenes (4): Q2: Do you think that you can make a dominant negative mutation of a membrane spanning receptor which function as a monomer? Circle one! (3) YES NO Briefly describe the reason why. (5) Q3: Briefly describe a difference between RSV and ALV with respect to latent periods that they need to induce tumors. (5) Q4: There are two sets of PCR primers (a and b) for human disease gene hunt. When we screened 50 DNA samples, the set (a) produced many different sized PCR products. On the other hand, the set (b) only produced two sized fragments. Which set is more useful as a molecular marker for human disease gene hunt? T4) Page 1 Q5: I proposed to examine differences in the first and the last exons of a human disease gene for genetic diagnosis. However, my proposal was rejected because it did not provide sufficient data for genetic diagnosis. Why? (5) Q6: Which molecular technique (or strategy) shall we use FIRST in the following circumstances? (4X5) (a) When you want to isolate a DNA fragment as a clone: (b) When you want to examine DNA structural differences among several mutations using a cDNA clone: (c) When you want to examine levels of expression of a particular gene using a cDNA clone: (d) When you want to test whether a lOObp long DNA fragment binds to a protein isolated from the nuclei: (e) When you want to determine which DNA fragment (those are 100 bp long and isolated from 5' regulatory region of a gene) is the most effective positive enhancer Q7: This is a cloning question!!! Organism of interest is Drosophila. You have a viable recessive mutation, whose mutant phenotype is wingless. This mutation was induced by a chemical mutagen called EMS, which only induces base substitutions, therefore this mutant allele is NOT suitable for cloning. What mutagen, do you choose when you induce more mutant alleles of thig gene? You MUST choose the one which DIRECTLY leads you to cloning of this gene. (3) Next, state steps (ONLY STEFgJlpjvaid cloning of this gene up to the final step with which vou really declare that you clon^e {hfc gene. (12) Pa°e2
