DNA Replication in E. coli and B. subtilis - Polymerase III, Fork Proteins, Exams of Molecular biology

Download DNA Replication in E. coli and B. subtilis - Polymerase III, Fork Proteins and more Exams Molecular biology in PDF only on Docsity! Exam 1 Lecture 5 1. In E. coli, how many catalytic subunits are used in the replication of both strands? How many in B.subtilis? 2. List the subunits of DNA Polymerase III holoenzyme in E. coli and know their functions. 3. How many forms of DNA Polymerase III are known from biochemical studies? 4. Which subunit acts as the dimerizing agent for DNA Polymerase III? 5. Which two subunits in DNA Polymerase III of E. coli contribute to its increased processivity? 6. What is the function of the  subunit of DNA Polymerase III of E. coli? 7. How many clamp loaders are present in the holoenzyme of DNA Polymerase III of E. coli? 8. Which two components of DNA Polymerase III of E. coli recognize primer template before assembling the holoenzyme? 9. Which two subunits of DNA Polymerase III of E. coli are encoded by the same gene? 10. Why is the holoenzyme of DNA Polymerase III of E. coli asymmetric? 11. How does clamp loader load  subunits of DNA Polymerase III of E. coli onto DNA? 12. List the proteins present at the oriC replication fork. What is the function of each protein? 13. What are the two properties of DnaB helicase at oriC? 14. List the proteins that constitute the primosome at oriC. 15. Which protein stimulates DnaB? 16. Which protein propels the replication fork at oriC? Which activates DnaG? 17. Which DNA polymerase pulls the lagging strand through during bacterial chromosomal replication?