Microbial Experiments: Catalase Production, Isolation, and Enzyme Reactions - Prof. G. Bri, Study notes of Biology

Download Microbial Experiments: Catalase Production, Isolation, and Enzyme Reactions - Prof. G. Bri and more Study notes Biology in PDF only on Docsity! Staphylococcus aureus Experiment 20 (catalase production) - aerobic -catalase positive Experiment 31 (isolation & ID staph) -normal flora -yellow on MSA -coagulase + -gram positive - ferments mannitol -pathogenic (produces enterotoxin) - thick plasma appearance -causes toxic shock -causes plasma to clot Staphylococcus epidermidis Experiment 20 (catalase production) -aerobic -catalase positive Experiment 31 (isolation & ID staph) -normal flora -does not ferment mannitol -few virulence factors -runny plasma appearance -white on MSA -coagulase - Experiment 33(symbiosis) -facultative anaerobe -removes oxygen so C. sporogenes can survive Micrococcus Experiment 20 (catalase production) -aerobic -catalase positive Lactobacillus Experiment 20 (catalase production) -catalase negative Experiment 28 (yogurt) -produce lactic acid -grow when pH drops below 6 -gram positive -aerotolerant anaerobes Lactococcus lactis Experiment 23 (fermentation of carbs) -did not ferment milk -pH 6 Saccharomyces cerevisiae Experiment 23 (fermentation of carbs) -produce acid and gas in both malt extract and grape juice Bacillus cereus Experiment 23 (fermentation of carbs) -growth and acid production in glucose and sucrose Streptococcus mutans Experiment 20 (catalase production) -fermentative -catalase negative Experiment 27 (isolantion & ID strept) -produce alpha hemolysin -green colonies -aka viridians -develop methemoglobin Streptococcus pyogenes Exp. 20 (catalase production) -fermentative -catalase negative Exp. 27 (isolation & ID strept) -strep throat -produces a beta hemolysin -scarlet fever -colonies have a clearing -produce streptokinase Enterococcus Exp. 20 (catalase production) -catalase negative Escherichia coli Exp. 21 (amylase production) -amylase negative Exp. 22 (caseinase & gelatinase) -caseinase & gelatinase negative Exp. 23 (fermentation of carbs) -growth, acid, & gas production for glucose & lactose Exp. 24 (cysteine desulfhydrase) -cysteine desulfhydrase negative Exp. 25 (IMViC) -indicator for fecal contamination -indole positive -VP negative -MR positive -citrate negative Exp. 26 (nitrate reduction) -no gas production -reduced to NO2 Exp. 30 (bacteriophage titration) -infected by phage lambda Bacillus subtillis Exp. 21 (amylase production) -amylase positive Exp. 22 (caseinase & gelatinase) -caseinase & gelatinase positive Citrobacter freundii Exp. 23 (fermentation of carbs) -growth, acid, & gas productions for glucose, lactose, & sucrose Exp. 24 (cysteine desulfhydrase) -cysteine desulfydrase positive Enterobacter aerogenes Exp. 25 (IMViC) -may be found in feces -MR negative -found in environment -VP positive -found in decaying vegetation -citrate positive -indole negative Paracoccus denitrificans Exp. 26 (nitrate reduction) -produced gas -reduced to N2 Clostridium sporogenes Exp. 33 (symbiosis) -obligate anaerobe