BIO 171 Lab 3 Notebook Mounting techniques (Portage learning), Lab Reports of Microbiology

Download BIO 171 Lab 3 Notebook Mounting techniques (Portage learning) and more Lab Reports Microbiology in PDF only on Docsity! Lab 3 Notebook Back to Home Page Title: PL#3 / Mounting Techniques Objective: Microscopic examination of bacterial samples through various techniques. Identifycolor and shape of given samples Procedure: Dry Mount: 1. Clean slide (70% ethanol) 2. Circle area on slide for easy location of specimen 3. Apply organism to slide: If from culture, use sterile loop to spread onto slide-If from plate, use sterile loop to pick colonies and mix with a drop of distilled water 4. Air dry at room temperature until all moisture has evaporated Wet Mount: 1. Clean slide (70% ethanol) 2. Circle area on slide with a wax/hydrophobic pen (keeps sample in place) 3. Apply organism on slide-If from culture, use sterile loop to spread onto slide-If from plate, use sterile loop to pick colonies and mix with a drop of distilled water 4. View under microscope-Wet mount is ideal for viewing the motility of an organism. Do not dry out Gram Staining 1. Clean Slide (70% Ethanol) 2. Apply organism to slide (Use sterile loop to spread one to three drops onto slide, into a thin film) 3. Allow to air dry 4. Fix organism to slide by passing three times through a flame, and do no overheat. 5. Flood the slide with crystal violet for 30-60 seconds 6. Rinse slide with water 7. Cover with grams iodine for 30-60 seconds 8. Rinse slide with water 9. Decolorize with alcohol 10.Rinse slide with water 11.Counter stain with Safranin for 30 seconds 12.Rinse slide with water, and blot dry 13.Examine under microscope