Biochemistry Laboratory I - Laboratory Report #6 | Chem 472, Lab Reports of Chemistry

Download Biochemistry Laboratory I - Laboratory Report #6 | Chem 472 and more Lab Reports Chemistry in PDF only on Docsity! Lab Report Outline Expt 6a (PCR) (20 pts total) Abstract (1)The abstract should concisely tell you the results of the experiment and why they are important. The results of the PCR and electrophoresis analysis. State the apparent PCR product sizes observed for each reaction and Introduction (1) Background material. Describe and diagram the desired target structure of the clones that you analysed. Experimental Procedures (2)Sufficient detail should be included so that a trained biochemist (not necessarily a naïve student) can reproduce your results. Note instances where your techniques differed from the protocols. Describe the details of the PCR experiments (primer sequences, reaction components, thermal cycling parameters). Results (3) Tables and figures that concisely present your findings For experiment 6 report The gel photograph. With a table listing strains or DNA templates and observed fragment sizes. The results should also be presented in text form, the tables and figures should be explained Discussion (2) Insights into what may have gone wrong, explanations of surprising results, interpretations of significance data. Interpret the results of the PCR experiments. For each template a describe your interpretaton of the fragment sizes in terms of the structure of the plasmid and bacterial attachement sites. References (1)