Bioinformatics Exam Questions: Needleman-Wunsch Algorithm and Global Alignment, Exams of Biotechnology

Download Bioinformatics Exam Questions: Needleman-Wunsch Algorithm and Global Alignment and more Exams Biotechnology in PDF only on Docsity! BIOINFORMATICS– GLOBAL SEQUENCE ALIGNMENT EXAM QUESTIONS WITH ANSWERS Which of the following is not a disadvantage of the Needleman-Wunsch algorithm? (THEY ASKING FOR AN ADVANTAGE). a)This method is comparatively slow b) There is a need for intensive memory c)This cannot be applied to genome-sized sequences d) This method can be applied to even large-sized sequences Reason: THEY ARE ASKING FOR AN ADVANTAGE.  Which is true about Bioinformatics? A. Mine information from the large databases B. Utilizes databases provided by NCBI (National Center for Biotechnology info) C. Uses BLAST tool (Basic Local Alignment Search) to compare sequences in target molecules D. You used it to compare EGFR sequence length to that of other animals in one of the questions in this test. E. All of the above  A search for “Human EGFR” on the UniProtKB website indicates that the length of consensus (canonical) sequence of Human EGFR is the same as that of Drosophila melanogaster. a.True (Please Indicate corresponding lengths) b.False (Indicate corresponding lengths). -human length: 1,210 AA -drosophila melanogaster: 1,426 AA  In searching for clinical variants of EGFR on the NCBI site (ClinVar Dataset; Refer to my PPs on Bioinformatics/ Clinical Variance), I found information depicted in Item line #37, regarding the location of EGFR gene and Pathogenic single nucleotide substitution (SNP) that can cause cancer in certain tissue. Which of the following did you see? a.Chromosome #7; Change of nucleotide base A (Reference allele) to G (Variant allele) b.Chromosome 17 & SNP of G to A. Which of the following does not describe a global alignment algorithm? a)In the initialization step, the first row and first column are subject to a gap penalty b) Score can be negative c)In the trace-back step, the beginning is with the cell at the lower right of the matrix and it ends at the top left cell d) The first row and first column are set to zero Reason: Only 1st block is set to zero in global alignment. In local alignment, the rows and columns are set to zero.