Download caracterisation of the collagene and more Cheat Sheet Biochemistry in PDF only on Docsity! University of Monastir Faculty of Sciences of Monastir Department of Chemistry MR SRCO2 Academic year 2022-2023 Collagen synthesis, characteristics and his applications 1 the origin of collagen and his characteristics the chemical modifications on the collagen molecule. Different methods of collagen synthesis Collagen applications Plan 1 2 3 4 2 The origin of collagen Different sources of collagen Cattle Swine Avian Marine fish 5 structure of collagen The sequence of amino acids forming an alpha chain (Repetition of a motif of 3 amino acids) Native collagen molecule (3 alpha chains assembled in a triple helix) Collagen fiber (association of collagen molecule) Glycine Prolyne Hydroxy prolyne C. Nolte, P. Mayer and B. F. Straub, Angew. Chem. Int. Ed., 2007, 46, 2101. Alpha chain Native collagen molecule Collagen fiber 6 The different types of collagen Collagen type I represents 90% of collagen in humans. It is the connective tissue of the bones, skin, tendons, and internal organs Collagen type II is responsible for strong cartilage resistance. collagen Type III is present in muscles, the wall of blood vessels and also in hematological tissues 7 The different collagen synthesis methods Collagen synthesis Extraction of collagen from the scales of the Carp Extraction of collagen from bovine skin 10 Scale pre- treatment • The scales of the crap are decalcified using the acid (EDTA). Collagen extraction • the scales are solubilized in an acetic acid solution containing pepsin, then a centrifugation step. Purification of the collagen • the solution is subjected to precipitation for 12 to 24 hours without agitation to allow optimal recovery of collagen Synthesis of collagen from the scales of the Carp 11 Bovine collagen comes from the skin of cows It is a type I and III collagen product It is used to promote gastro-intestinal and articular health Synthesis of bovine collagen 12 collagen applications The Cosmetics field Moisturizer Anti-wrinkle Antioxidant Aesthetic medicine Microdermabrasion Radiofrequency Ultrasound and laser resurfacing Food industry Biomedical uses Gelatine Found in absorbable haemostatic sponges. J.S. Quesnel, B.A. Arndtsen. Pure Appl. Chem. 2013, 85, 377–384 15 Collagen plays an important role in the functioning of most of the structures in our body and in the health of our joints i i l i The collagen is a biopolymer, constituted by triple polypeptide chains wound in a triple helix. It is present in the extracellular matrix of animal organisms. . conclusion The function of these proteins is to give tissues mechanical resistance to stretching i i 16 Thank you for your attention 17
