Download Catalase Experiment and more Study notes Biology in PDF only on Docsity! Biology Partnership Grant Name: _______________________ Date: ______________________ Class Period: _______ CATALASE EXPERIMENT Background Information: One important class of proteins that is worth studying are enzymes. Enzymes are vital to almost all biological processes that changes in their environment can directly affect their effectivity. Enzymes are simply biological catalysts. It being a catalyst means that it increases the rate of a biochemical reaction specifically, after it has lowered the activation energy required to start up the reaction. In living organisms, a drastic and even the slightest change in factors such as temperature and pH can render an enzyme to become ineffective. Catalase is an enzyme that is present in virtually all parts of an animal’s body. It is contained in cells, produced by the organelle peroxisome. A high concentration of catalase is usually found in liver. This enzyme will break down hydrogen peroxide into water and oxygen upon contact. Objective: To observe the effect of changes in temperature and pH in enzyme activity. Materials: raw chicken liver, hydrogen peroxide solution, distilled water, beakers, graduated cylinder, blender, hot plate and concentrated hydrochloric acid Procedure: 1. Get a piece of raw chicken liver and put it inside a blender. Add 100 mL of water and then have the mixture blended until a ”liver juice” is created. 2. Divide the liver juice into 3 approximately equal parts by pouring it into 3 separate beakers. 3. Label the first one as the control and the other two as Mixtures 2 and 3 respectively. 4. In each of the 3 beakers, put 10 mL of hydrogen peroxide solution. Label each of these as Solutions 1, 2 & 3 respectively. 5. Mix Solution 1 with the control and observe what will happen. 6. Heat Mixture 2 on low hot plate setting and remove it when a distinct color change has occurred. Let the mixture cool down for 5 ~ 10 minutes. Pour Solution 2 afterwards and observe what will happen. 7. Mix 10 mL of concentrated hydrochloric acid with Mixture 3. Stir the resulting mixture and then pour Solution 3 and observe what will happen. Data Presentation & Analysis A. Data Table Mixture Effect of Adding Hydrogen Peroxide Solution (Observations) Mixture 1 (original mixture) Mixture 2 (heated mixture) Mixture 3 (acidified mixture)
