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Catalase Detection in Microbiology Lab: Experiment 5-4 with Various Bacteria - Prof. Shawn, Lab Reports of Microbiology

Montgomery College Microbiology

Prof. Shawn Lester

The procedure for conducting a microbiology lab experiment to test for the presence of the enzyme catalase in enterococcus fecalis, staph. Aureus, m. Luteus, and b. Spp. Using the slide test method with hydrogen peroxide. Learn about the role of catalase, the reaction it catalyzes, and the significance of this enzyme for different groups of organisms.

Typology: Lab Reports

Pre 2010

Uploaded on 03/10/2009

koofers-user-tse 🇺🇸

10 documents

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Download Catalase Detection in Microbiology Lab: Experiment 5-4 with Various Bacteria - Prof. Shawn and more Lab Reports Microbiology in PDF only on Docsity! Microbiology Lab Experiment Changes Experiment #: 5-4 Title: Catalase Live Organisms: Enterococcus fecalis, Staph. aureus, M. luteus, B. spp. Changes: Slide Test Method 1. Obtain a glass slide. 2. Put a drop of hydrogen peroxide on the slide. 3. Obtain an inoculum of bacteria using a sterile loop and mix the bacteria into the peroxide. Alternately, you may put the bacteria on the slide first then add a drop of peroxide. Either way works. 4. Observe for the presence of bubbles. 5. Do Not put peroxide on the slant! Note: Bacillus spp. sometimes are not to be strong catalase producers. Use a heavy inoculum of this organism. Take Home Lesson: See Experiment 30 in lab manual for additional background information. We are testing for the presence of the enzyme catalase. What reaction does catalase catalyze? Which groups of organisms possess catalase? What does superoxide dismutase do? Why is oxygen toxic to strict anaerobes?

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