Download Chromatography and Electrophoresis - Laboratory Report | Chem 472 and more Lab Reports Chemistry in PDF only on Docsity! Lab Report Outline Expt 3 (Chromatography) & Expt 4 (Electrophoresis) (20 pts total) Abstract (2) The abstract should concisely tell you the results of the experiment and why they are important. For experiment 3 report The elution volumes of Blue Dextran, B12 and heme proteins on your GF column. The overall recovery and relative purity (units/mg of total protein) of Beta galactosidase in your elution fractions. For experiment 4 report. The fractionation pattern from the SDS Gel. And the estimated molecular weights of the unknown proteins Introduction (2) Background material Experimental Procedures (4) Sufficient detail should be included so that a trained biochemist (not necessarily a naïve student) can reproduce your results. Note instances where your techniques differed from the protocols. Describe in detail the chromatography steps that you used, the technique for pouring loading, running and staining the gel.. Results (6) Tables and figures that concisely present your findings For experiment 3 report The elution volumes of Blue Dextran, B12 and heme protein on your GF column in table format. Your metal affinity result, including a table with the yield, purity of your protein in each of the elution fractions For experiment 4 report A picture of the stained SDS Gel and a comparison of the gel result with the total protein and enzyme assay results above. A graph of the relative mobilities of the standard proteins(BSA-67 kDa; Pepsin-35 kDa ; Hemoglobin 16.5 kDa and your unknown sample, and an estimate of its molecular weights. The results should also be presented in text form, the tables and figures should be explained Include answers to questions asked in the text that are directly answered by your data. Discussion (4) Insights into what may have gone wrong, explanations of surprising results, interpretations of significance data. Include answers to questions asked in the text that are more speculative. References (2)
