CTBS Exam Review With Complete Questions And Answers 100% Verified, Exams of Nursing

Download CTBS Exam Review With Complete Questions And Answers 100% Verified and more Exams Nursing in PDF only on Docsity! 1 CTBS Exam Review With Complete Questions And Answers 100% What is an automatic deferral for heart valve donation? history of cancer obesity cardiomyopat hy idiopathic disorders - Correct Answer-Cardiomyopathy AATB standards require that tissue banking maintain SOPs encompassing all of the following except: a. donor suitability criteria and record management policies b. FDA regulations that apply to tissue banking c. procedures for monitoring in-process controls d. procedures describing criteria for exceptional release of tissue - Correct Answer-FDA regulations that apply to tissue banking During skin processing, what is the maximum amount of time that skin can be exposed to temperatures above 10 degrees Celsius? 2hr s 1hr 8hr 4hr - Correct Answer-2 hours At what temperature shall cardiovascular tissue be stored? -50 degree celsius or colder -1 degree celsius or colder -25 degree celsius or colder -100 degree celsius or colder - Correct Answer--100 degree Celsius or colder What must be considered for pediatric heart donors that have been breastfed in the 12 months prior to donation? a. length of time donor was fed b. mother's blood must be used for serology testing c. analysis of mother's risk for transmissible disease d. analysis of mother's nutritional habits - Correct Answer-Analysis of mother's risk for transmissible disease Pre-processing cultures are a. used to detect pathogenic yeast, viruses, and bacteria b. required by AATB standards 1 c. reviewed by the medical director designee d. preformed immediately after tissue exposure to antibiotics - Correct Answer-required by AATB standards 1 For in process controls, what percentage of grafts must be swabbed for testing? 100% 20% 10% 50% - Correct Answer-100% What is the maximum amount of time that a donor can remain unrefrigerated prior to the commencement or recovery? 24hr s 12hr s 15hr s 8hrs - Correct Answer-15 hours Which of the following is a cryoprotectant a. isotonic saline b. Glycerol c. RPMI d. Typticase soy broth - Correct Answer-Glycerol The upper time limit from retrieval to processing of heart valves is a. there is no time limit as long as aseptic technique is maintained during the recovery b. total ischemic time must not exceed 48hrs c. cold ischemic time must not exceed 48hrs d. warm ischemic time must not exceed 48hrs - Correct Answer-Total ischemic time must not exceed 48hrs All tissues not already transplanted, which are subject to recall, shall be located and quarantined pending resolution of the recall (T/F)? - Correct Answer-True Which of the following is not a colloid solution? fresh frozen plasma ringers lactate dextran albumin - Correct Answer-Ringers Lactate What is the most common STD in the US? HIV Syphilis Genital herpes Gonorrhea - Correct Answer-Genital herpes 1 What is the most common bloodbourne pathogen in the US today? HIV Hep B Hep A Hep C - Correct Answer-Hepatitis B 1 The lesser trochanter is a landmark on the tibia ulna femu r humerus - Correct Answer-Femur What is the minimum classed environment needed for processing of cardiovascular tissue? a. not applicable b. class 10 or cleaner c. class 500 or cleaner d. class 100 or cleaner - Correct Answer-class 100 or cleaner What tissue transplant is most closely associated with CJD? Iliac crest Femur Dura Mater Skin - Correct Answer-Dura Mater Serological testing on a potential donor reveals a positive HIV antibody screening test. The screening test is positive on repeat testing, but the confirmatory test result is negative. What happens to the tissue. a. may not be used; discrepant results obtained b. may be used; HIV antibody test is not a required test c. may be used; confirmatory test is negative d. may not be used; results of confirmatory test cannot override a screening test - Correct Answer-may not be used; results of confirmatory test cannot override a screening test Which of the following is not a cause for deferral? a. A positive PPD test for TB b. untreated syphilis c. systemic mycosis d. evidence of sepsis - Correct Answer-A positive PPD test for TB Which of the following transplants is most commonly associated with spread of prion related disease? a. skin b. dura mater c. bone 1 every 72hrs every 12hrs - Correct Answer-Every 72hrs Wolf's Law describes a. fracture repair in long bones b. the amount of pressure a bone can take before fracture 1 c. the relationship between form and function in bone. d. bone regeneration - Correct Answer-Bone regeneration AN example of the immune response is a. Low pH on the skin b. itching from a bee sting c. normal flora of the intestines d. flushing action of tears - Correct Answer-Itching from a bee sting Which of the following would be used for an osteochondral graft? a. patellar tendon b. tri-cortical iliac wedge c. crushed cancellous/ cortical chips d. distal femur - Correct Answer-Distal femur At what temp should hearts be transported to the processing facility? 4 degree celsius 1-10 degree celsius 2-10 degree celsius temp doesn't matter as long as the heart is on ice - Correct Answer-1-10 degree celsius Which organism, if present, requires discarding of skin grafts? a. Staphylococcus sicuri b. Streptococcus sanguis c. Streptococcus viridans d. Staphylococcus aureus - Correct Answer-Staphylococcus Aureus Cryopreserved allograft saphenous veins are NOT used in which procedure? a. AV fistulas for use as dialysis conduits b. peripheral vascular surgery c. temporary limb revascularization in traumatic vessel loss. d. carotid endarterectomies - Correct Answer-Caroted Endarterectomies Quality control is described by all of the following EXCEPT a. is one dimension of quality assurance b. provides for the creation of standard operating procedures c. provides microbiological testing of tissue at pre-sterilization and at final packaging stages. d. provides tests to monitor specific processes - Correct Answer- Provides for the creation of standard operating procedures Is Albumin a crystalloid or colloid? - Correct Answer-Colloid Which of the following organisms found on pre-processing culture from bone 1 shall be a deferral of that tissue? a. Nuisancesis kasprinis 1 c. to rid the skin of all microbes d. to sterilize the patient - Correct Answer-To rid the skin of most microbes The right ventricular outflow tract contains thevalve and conduit and the left ventricular outflow tract contains the valve and conduit. a. tricuspid, mitral b. aortic, pulmonary c. mitral, tricuspid d. pulmonary, aortic - Correct Answer-pulmonary/ aortic The most probable origin of bacteria contaminating a heart procurement is a. lungs b. skin c. upper respiratory tract d blood - Correct Answer-Lungs Optimal freezing rate for allograft heart valve is a. 1 deg celsius/ minute b. 5 deg celsius/ second c. 5 deg celsius/ minute d. 1 deg celsius/ second - Correct Answer-1 degree celsius/ minute Total ischemic time in heart for valve recovery should not exceed 24hrs 36hr s 12hr s 48hrs - Correct Answer-48hrs What is the maximum time recovered musculoskeletal tissue shall remain at wet ice temp before either processing or freezing? 12hr s 15hr s 72hr s 24hrs - Correct Answer-72hrs Processing and packaging of cardiovascular grafts must take place under a. class 100/ 1000 conditions b. class 1000/100 conditions c. class 100/ 100 conditions 1 d. class 1000/ 1000 conditions - Correct Answer-class 100/ 100 conditions To grow on laboratory media, anaerobic organisms require a. reduced oxygen b. increased oxygen c. reduced nitrogen d. increased hydrogen - Correct Answer-Reduced oxygen 1 One goal of cardiovascular tissue banking is to preserve the of the leaflet and the of the valve matrix. a. viability/ integrity b. viability/ rigidity c. volume/ area d. solubility/ permeability - Correct Answer-Viability/ Integrity The most common means of sterilization for musculoskeletal tissue is the use of a. Ethylene oxide b. Ionizing radiation c. carbon dioxide d. pressurized steam - Correct Answer-Ionizing Radiation When retrieving the achilles tendon with a bone block, the bone block is a. the tarsus b. the calcaneous c. the tibial tuberosity d. the fibular notch - Correct Answer-the calcaneous Which bone(s) is not in the foot? a. Metacarpal bones b. Metatarsal bones c. phalanges d. lateral cuneiform bone - Correct Answer-Metacarpal Bones Early data suggests that cryopreservation of the cardiac allografts made them superior to both porcine and mechanical valves in terms of freedom from and . a. blood thinners/ carbon plated support ring b. dysfunction/ noise c. re-operation/ infection d. degeneration/ high cost - Correct Answer-blood thinners/ carbon plated support ring Is TPN a crystalloid or colloid? - Correct Answer-Crystalloid When retrieving the femur and tibia, you should start your incision a. anteriorly over the patellar region b. at the anterior- superior iliac spine c. at the lateral aspect of the femur d. at the greater trochanter - Correct Answer-At the anterior- superior iliac spine 1 What is the shelf life for tissue stored at temperature below -40 deg celsius? a. 1yr unless a longer expiration date has been validated b. 5 yrs unless a longer expiration date has been validated c. not applicable d. 20 yrs unless a longer expiration date has been validated - Correct Answer-5 yrs unless a longer expiration date has been validated. 1 A patient has a fever, elevated white count and positive blood cultures for E.coli, according to AATB standards what action should be taken with musculoskeletal tissue? a. accept bone, defer tendons b. accept, but radiate c. accept d. defer - Correct Answer-Defer All of the following are advantages of using allograft heart valves except a. non-obstructive flow b. catastrophic failures are rare c. anticoagulation therapy is not needed d. supply is unlimited compared to xenographs - Correct Answer-supply is unlimited compared to xenographs Which best describes the flow of blood through the heart? a. right atrium, right ventricle, pulmonary artery, pulmonary vein, left atrium, left ventricle b. left atrium, left ventricle, pulmonary vein, right atrium, right ventricle c. right atrium, right ventricle, pulmonary vein, pulmonary artery, left atrium, left ventricle. d. left atrium, left ventricle, pulmonary artery, pulmonary vein, right atrium, right ventricle - Correct Answer-Right atrium, right ventricle, pulmonary artery, pulmonary vein, left atrium, left ventricle Which of the following is NOT an adequate transport solution characteristic for the recovered heart? a. Isotonic b Cryoprotectan t c. Cold d. Sterile - Correct Answer-Cryoprotectant Which of the following is not a structural allograft? a. femoral cortical strut b. iliac crest wedge. c. cloward dowel d. ground corticocancellous - Correct Answer-Ground corticocancellous You have consent for skin and musculoskeletal tissue, but the donor has rheumatoid arthritis. According to AATB standards, is the donor a deferral? a. no, skin could be recovered b. yes, musculoskeletal tissue can be recovered c. no, musculoskeletal tissue can be recovered 1 d. AATB has no guidance in this tissue - Correct Answer-No, skin could be recovered During heart valve dissection, processed cardiac tissue is placed in antibiotic solution, resulting in a graft. 1 b. to prevent intercellular ice formation c. to prevent extracellular ice formation d. to minimize osmotic imbalances - Correct Answer-To prevent extracellular ice formation AATB standards require that tissue processing be preformed by a. CTBS technicians b. staff member with a medical background c. staff member with sufficient background, experienced and training d. staff member with 2-4 yr degree - Correct Answer-Staff member with sufficient background, experiences and training Which of the following is used for quality control of gamma irradiation? a. bacillis subtilis spore strips b. bacillis pumilis spore strips c. bacillis cereus spore strips d. chemical indicator sport strips - Correct Answer-Bacillis subtilis spore strips Donors with a history of exposure to or ingestion of toxic substances should be. a. deferred as a tissue donor b. accepted as a tissue donor depending on the ID of the substance c. considered as suitable if the tissue bank specialist adheres to universal precautions d. evaluated by the medical director for transplant suitability - Correct Answer-Evaluated by the medical director for transplant suitability A donor has systemic lupus, according to AATB standards, which of the following tissues must be deferred? a. femoral artery b. achilles tendon c. heart valves d. skin - Correct Answer-Skin The 2 heart valves most often used in cardiac surgery are the a. mitral, tricuspid b. ventricle, atrium c. pulmonary, aortic d. bicuspid, mitral - Correct Answer-Pulmonary and Aortic AATB requirements for serological testing include all of the following except a. HBsAg b. RPR c HIV 1 I&II d. HIV Ag - Correct Answer-HIV Ag Which of the following diseases is caused by a spirochete? a. HCV 1 b. Syphilis c. CJD d. HIV - Correct Answer-Syphilis Which of the following is not a deferral criterion for cardiovascular tissue? a. Diabetes Mellitus b. Indiopathic Cardiomyopathy c. Rheumatic fever d. Bacterial Endocarditis - Correct Answer-Diabetes Mellitus What is the name of the incision used for musculoskeletal tissue recovery? a. Harris approach b. McFarland & Osborne approach c. Modified Watson Jones d. Hardinge Approach - Correct Answer-Modified Watson Jones All of the following vessels are transected during heart for valve recovery except a. Left coronary artery b. Left common carotid c. Brachiocephalic d. Left subclavian - Correct Answer-Left Coronary artery What was the first established tissue bank? a. university of fla tissue bank b. US naval tissue bank c. university of miami tissue bank d. US FDA tissue bank - Correct Answer-US Naval tissue bank Over what age is an algorithm no longer necessary if there is no bleed and the donor has been infused? a 10 b 12 c 14 d 8 - Correct Answer-12 Retrieved musculoskeletal tissue will be wrapped aseptically with at least moisture barriers? 2 4 1 d. cancellous - Correct Answer-Semitendinosis What is the primary healing mechanism after allograft implantation? a. osteoclastic degeneration b. re-vascularization c. osteoblastic regeneration d. creeping substitution - Correct Answer-Creeping substitution 1 Which of the following diseases is probably caused by a prion? a. HTLV-1 b. CJD c. HIV d. Hep C - Correct Answer-CJD Which of the following tests are not required by AATB? a. HIV p24 anitgen b. anti- HTLV-1 c. HIV NAT d. anti-HIV -1 - Correct Answer-anti-HTLV-1 Is HIV/NAT testing required by AATB standards? (Y/N) - Correct Answer-Yes The warmest temp allowable for storage of cryopreserved heart valve is a. -100 deg celsius b. -80 deg celsius c. -196 deg celsius d. -40 deg celsius - Correct Answer--100 deg celsius You have recovered the heart valves from a 4 month old who died as a result of SIDS. In SIDS cases, an autopsy must be performed to determine a. none of these b. true age of donor c. time of death d. the cause of death - Correct Answer-None of these The prefix of a medical term will usually tell you a. the action b. the condition c. the part effected d. the location - Correct Answer-The part effected What is the term for cells and/or tissues intended for transplant into another individual of the same species? a. tissue b. zenogra ft c. autograft d. allograft - Correct Answer-Allograft The technique implementing the use of a unicortical dowel for spinal 1 infusions is referred to as the a. cloward procedure b. smith-jones procedure c. smith- barr procedure 1 d. endocardium, myocardium exocardium - Correct Answer-Endocardium, myocardium, epicardium What muscle is transected at the quadriceps tendon during the tissue recovery? a. vastus quadralis b. rectus femoris c. iliotibial band d. semitendinosis - Correct Answer-Rectus femoris Potential donors who have received pituitary derived human growth hormone should be excluded from tissue donation due to the possibility of transmission of a. dwarfism b. alzheimer's disease c. encephalitis d. CJD - Correct Answer-Encephalitis How long can skin be stored refrigerated? 48hrs 14 days 24hrs 7 days - Correct Answer-14 days Assuring and verifying confidence in the quality of the process used for finished tissue, and documenting that the entire process conforms with AATB standards for tissue banking is called a. quality control b. quality assurance c quali- temps d quality systems - Correct Answer-Quality assurance During heart for valve recovery, which of the following vessels should be left at maximum length? a. Pulmonary artery b. Pulmonary vein c. superior vena cava d. inferior vena cava - Correct Answer-Pulmonary vein Quality assurance is required to investigate all adverse outcomes and a. employee complaints of unfair treatment b. employee complaints regarding a client c. employee complaints of sexual harassment 1 d. employee accidents - Correct Answer-Employee accidents A potential donor's history includes rheumatoid arthritis that required prolonged systemic steroid use. You should a. call the family physician 1 b. proceed with recovery c. defer the donation d. get medical director opinion - Correct Answer-Defer the donation In what instance would an elevated WBC count no necessarily indicate an infectious process? a. long term ventilator assisted breathing b. head trauma c. prolonged elevated body temp d. pneumonia - Correct Answer-Head trauma Documentation of a telephone consent with the next of kin must include a. recording or monitoring by the requestor and a witness b. self signed donor document or photocopy c. telegram documenting next of kin consent d. signature of next of kin and a witness - Correct Answer-Recording or monitoring by the requestor and a witness An agent that kills microorganisms on inanimate objects is a. a disinfectant b. a bacteriostat c. an antiseptic d. a sterilizer - Correct Answer-a disinfectant For what serological test may a confirmatory test be used to override a screening test result? a. RPR b. CMV c. HIV I & II d. HTLV I & II - Correct Answer-RPR Examination of the body should look for evidence of which of the following? a. all of these b. lymph nodes c. genital ulcerative disease d. oral thrush - Correct Answer-All of these All of the following allografts can be used for ACL repairs except a. Patellar tendon b. achilles tendon c. 1 d. the time from infection to a detectable level - Correct Answer-The time form infection to a detectable level According to AATB standards, refrigerated skin can be stored a. at 10 deg celsius for 12 days b. at 14 deg celsius for 10 days 1 c. at 8 deg celsius for 16 days d. at 12 deg celsius for 14 days - Correct Answer-At 12 degrees celsius for 14 days If a chart review of potential donor who has expired from a gun shot wound to the head reveals a WBC count of 31,000 you should a. take note of the results and report them to the medical director b. decline the donation, the high WBC indicated infection c. take note of the WBC count and decline the donation unless the tissue is urgently needed d. ignore this value since this is often seen in head injuries - Correct Answer- Ignore this value since this is often seen in head injuries According to AATB standards, monetary compensation of a donor may be given when a. incentives will influence loved ones to donate b. donor families need funds for burial expenses c. a donor's estate request it d. living donors incur costs for their donation - Correct Answer-Living donors incur costs for their donation A donor was last seen by spouse at 6pm, phoned home at 7pm, flushed toilet at 8pm was found asystolic by ambulance staff and pronounced at 11pm. What time is used for last know alive? 11p m 8pm 6pm 7pm - Correct Answer-8pm There are approximately cubic centimeters in one fluid ounce 16 36 12 30 - Correct Answer-30 At what temp should frozen musculoskeletal tissue be stored for >6months? a. -20 deg celsius or colder b. -40 deg celsius or colder c. -60 deg celsius or colder d. -10 deg celsius or colder - Correct Answer--40 deg celsius or colder Cancellous bone is composed of a. nonmyelinated fibers that are radiating in all directions b. thin process of trabeculae joined together forming a 3 dimensional lattice work c. Inactive osteoclasts, fibrocartilage, and fibromectin 1 d. cylindrical layers of haversian canals - Correct Answer-Thin processes of trabeculae joined together forming a 3 dimensional lattice work 1 Which of the following results in automatic deferral? a. repeat reactive anti-HIV-1 b. repeat reactive HBsAg c. Initially reactive anti-HTLV-1 d. confirmed positive syphilis - Correct Answer-All these are correct: repeat reactive anti-HIV-1 repeat reactive HBsAg initially reactive anti- HTLV-1 When preserving grafts by controlled rate freezing, which of the following must be closely monitored? a. temp change over time b. time at which -40 deg celsius is reached c. latent heat of fusion d. package integrity - Correct Answer-Latent heat of fusion Which of the following allograft types is not used in total hip revision? a. femoral head b. distal femur c. proximal osteoarticular d. proximal head - Correct Answer-Proximal osteoarticular AATB allows for the release of tissue without complete tissue results. In such circumstances, all of the following documentation is required except a. end user waiver form b. medical director release c. documented clinical need d. recipient approval - Correct Answer-Documented clinical need What procurement technique best reduces the risk of transport bio-burden (transporting microorganisms with the heart)? a. keeping the heart at required temp after recovery b. surgical hand scrub c. thorough rinsing of the heart with sterile solution d. shortened transport time - Correct Answer-Thorough rinsing of the heart with sterile solution When preparing a donor, the prep is generally performed. a. in a uniform fashion regardless of body area b. on "clean" body areas only c. from "clean to dirty" body areas d. from "dirty to clean" body areas - Correct Answer-From "clean to dirty" body areas 1 Donor suitability records for dura mater donors shall be retained for what period of time? a. indefinitely b. not a requirement 1 c. 1 yr d. 5yrs - Correct Answer-indefinitely Bone resulting from the use of demineralized human graft materials probably forms by a. Autogenesis b. Guided tissue regeneration c. Endochondral formation d. Intramembranous formation - Correct Answer-Guided Tissue regeneration Which of the following statements about disease transmission is false? a. heart valves commonly transmit bacterial infection b. corneas can transmit CJD c. tuberculosis infection from transplanted kidneys is an emerging problem d. corneas are poor transmitters of microorganisms - Correct Answer- Tuberculosis infection from transplanted kidneys is an emerging problem Skin and musculoskeletal recovery shall commence within how many hours of asystole if the body was not refrigerated 8hrs 4hrs 15hr s 12hrs - Correct Answer-15hrs On what date did the GTPs go into effect? a. May 25, 2204 b. May 14, 2004 c. May 14, 2005 d. May 25, 2005 - Correct Answer-May 25, 2005 If a donor is in the prone position, is the donor face up or face down? - Correct Answer- Face down According to AATB standards, how often must a comprehensive internal audit be done? a. quarterly b. annually c. monthly d. semi-annually - Correct Answer-Annually The Ross procedures uses pulmonary heart valve allografts to replace. a. the mitral valve, which is then used to replace the semilunar valve b. the aortic valve, which is then used to 1 antibiotics b. may be recovered if normal cryopreservation techniques are used c. may never be recovered d. may be recovered if it has been submerged in alcohol for 10min prior to wrapping - Correct Answer-May never be recovered 1 Refrigerated MS tissue must be retrieved aseptically and placed in an isotonic or nutrient medium with suitable antibiotics at no more than a. 15 deg C b. 20 deg C c. 25 deg C d. 10 deg C - Correct Answer-10 deg C Age limits for MS tissue donation are determined by a. the requesting physician b. the donor family c. the medical director d. the medical history of the donor - Correct Answer-The medical director The major weight baring bone between the ankle and the knee is the a. tarsus b. fibula c. femur d. tibia - Correct Answer-Tibia Bone resulting from the use of demineralized human graft materials probably forms by a. endochondral formation b. guided tissue regeneration c. intramembranous formation d. autogenesis - Correct Answer-Guided tissue regeneration Cancellous bone is composed of a. nonmyelinated fibers that are radiating in all directions b. thin process of trabeculae joined together forming a 3 dimensional lattice work c. tarsus d. inactive osteoclasts, fibrocartilage, and fibronectin - Correct Answer-Thin process of trabeculae joined together forming a 3 dimensional lattice work Which of the following cells is the most prevalent in the remodeling of bone a. osteoclasts b. osteocytes c. mesenchymal cells d. osteoblasts - Correct Answer-Osteoblasts Massive intercalary allografts are used primarily for a. bone induction process b. structural support in long bones c. placing in infected 1 wounds d. grinding into chips for filling defects - Correct Answer-Structural support in long bones When recovering the achilles tendon with a bone block, the bone block is a. the calcaneous 1 When recovering the femur, the incision should begin a. anteriorly over the patellar tendon b. at the anterior-superior iliac spine c. at the lateral aspect of the femur d. at the greater trochanter - Correct Answer-at the anterior-superior iliac spine Osteochondral allograft refers to bone that has been treated primarily to cryopreserve the a. haversion system b. cartilate of articular surfaces c. cortical bone matrix d. bone morphogenic proteins - Correct Answer-Cortical bone matrix If any additional processing of refrigerated MS tissue is necessary, the total wet ice/refrig time must not exceed from time of retrieval a. 5 days b 72hrs c. 24hrs d 4 days - Correct Answer-72hrs Which of the following cell types is the most mature? a. Howships' lacunae b. osteocyt es c. osteoblast d. osteoclast - Correct Answer-Osteocytes If the donor body has not been refrigerated, tissue excision must begin within hrs of asystole a. 15 b. 24 c. 12 d. 10 - Correct Answer-15 Skin should be procured to depth of a. 6-11 thousands of an inch b. 19-35 thousands of an inch c. 1-5 thousands of an inch d. 12-18 thousands of an inch - Correct Answer-12-18 thousands of an inch 1 Which of the following medications could possibly mask an infection process and may contribute to thin, brittle skin? a. antihypertensive b. topical lotions containing para amino benzoic acid c. long term, high dosages corticosteroids d. anti-depressants - Correct Answer-Long term, high dosages corticosteroids 1 When skin grafts are removed, the use of tissue culture media such as RPMI or DMEM is a. selected because they contain a pH indicator not present in normal saline b. because of the nutrient support it provides to the skin cells c. due to the unique dilutent properties they possess with glycerol for freezing d. because of nutrient support for the stratum corneum - Correct Answer- Because of the nutrient support it provides to the skin cells In order to maintain optimal cellular viability, skin grafts may be maintained prior to freezing. a. in sterile water b. in tissue culture media at 1 deg C to 10 deg C c. at 25deg C d. indefinitely at 4 deg C - Correct Answer-in tissue culture media at 1 deg C to 10 deg C While shaving the donor for skin recovery, the technician: a. mush shave only if the donor is very hairy b. should shave carefully to remove all hair that may trap bacteria or debris in the graft area. c. should use only an electric razor to avoid nicks in the skin. d. need not be concerned about nicks as they will be removed during processing - Correct Answer-should shave carefully to remove all hair that may trap bacteria or debris in the graft area Following skin recovery, the tissue a. should be maintained at -40deg C until placed in liquid nitrogen. b. must be placed into cryopreservation media immediately to prevent cellular damage c. must be immediately placed into sterile solution d. should be soaked in antibiotic solution - Correct Answer-must me immediately placed into sterile solution Split thickness allograft skin may be used for all of the following EXCEPT a. testing the suitability of the wound bed for autografting b. permanent coverage of small full thickness wounds. c. overlay of widely meshed skin d. temporary coverage of excised wounds - Correct Answer-permanent coverage of small full thickness wounds Which of the following statements regarding rewarming of skin is NOT true? a. a major goal is the maintenance of tissue viability b. the temp of the rewarming bath should be 10-37 deg c. it should be removed from it's package prior to rewarming d. significant structural damage can occur during rewarming - Correct Answer-it should be removed from it's package prior to rewarming 1 d. Medical director - Correct Answer-medical director Skin damaged by scraping or rubbing a. bruise b. cut c. abrasion d. avulsion - Correct Answer-Abrasion Small, dark, sometimes raised congenital growth on the human skin, sometimes with a hair or hairs growing from it a. wart b. mole, nevus c. pimple d. birthmark - Correct Answer-Mole, nevus A swelling on the skin that contains fluid a. contusion b. nevus c. mole d. blister - Correct Answer-Blister On average adult skin covers square feet a. 40 b. 19 c. 20 d. 15 - Correct Answer-19 Cold ischemic time for vascular tissue is defined as: a. The time interval from systole to subjecting cardiac tissue to cold rinse (or transport) solution at retrieval b.The time interval from subjecting vascular tissue to transport solution and wet ice temp at retrieval to the beginning of disinfection c. The time interval from systole to subjecting vascular tissue to transport solution and wet ice temp at retrieval. d.The time interval from subjecting cardiac tissue to cold rinse (or transport) solution at retrieval to the beginning of disinfection - Correct Answer-The time interval from subjecting cardiac tissue to cold rinse (or transport) solution at the retrieval to the beginning of disinfection Which of the following are NOT considered to be pathogenic, highly virulent microorganisms that result in skeletal tissue discard: a. Sabouraud's Dextrose Agar b. Staphylococcus aureus c. Enterococcus sp 1 d. gram negative bacillis e. Strepococcus pyogenes 1 f. fungi (yeast/molds) - Correct Answer-Sabouraud's dextrose Agar To prevent additional warm ischemia and potential cellular or matrix damage caused by temp cycling, methods shall be employed that maintain the tissue or solutions above 1- 10 deg for longer than a. 24hrs b. 2 hrs c. 1hr d. 48hrs - Correct Answer-2 hrs Residual moisture analysis shall not exceed % of initial weight by nuclear magnetic resonance spectrometry a. 8% b. 6% c. 2% d. 10% - Correct Answer-8% What is the storage conditions temp for commonly transplanted human tissue: cardiac and vascular a. 1-10 deg C b. -100 deg C c. 10- 10 deg C d. -20to 4-10 deg C - Correct Answer--100 Deg C Processing, which includes dissection and packaging, of cardiac and vascular tissue shall be preformed in a certified and qualified Class ( or cleaner) laminar flow a. 1000 b. 500 c. 100 d. 200 - Correct Answer-100 To prevent additional warm ischemia and potential cellular or matrix damage caused by temp cycling, methods shall be employed that maintain the tissue and solutions a. 1-40 deg C b.1-10 deg C c. -1- -5 deg C d. 5 -10 deg C - Correct Answer-1-10 deg C Reagents shall be stored in accordance with AATB and FDA instructions (T/F) - Correct Answer-True 1 Vascular grafts shall be inspected, evaluated, and sized by diameter and recorded in . a. Millimeters (mm) b. not applicable c. Centimeters (cm) 1 d. inches - Correct Answer-centimeters (cm) The processing of osteoarticular tissue shall be completed within days of recovery a. 3 b. 5 c. 10 d. 14 - Correct Answer-5 days What are the acceptable residual levels of ethylene oxide for medium sterilized bone grafts? a. 100 b. 25 c. 250 d. 2,500 - Correct Answer-100 Musculoskeletal tissue shall be processed in a bacteriological and climate controlled environment (T/F) - Correct Answer-True What is the shelf life for tissue stored at temp below - 40 deg C a. unlimited b. 2yrs from date of processing c. 5yrs from date of processing d. 15 yrs from the date of processing - Correct Answer-5yrs from the date of processing What are the acceptable residual levels of ethylene glycol for small bone grafts? a. 2,500 b. 100 c. 250 d. 25 - Correct Answer-2,500 The most common sources of radiation are: a. Cobalt 60 b. Bio- cleanse c. X-ray d. Cobalt 40 e. Tutoplast f. Electro Beam - Correct Answer-Cobalt 60, X-ray, Electro Beam The length of the aortic conduit, main pulmonary artery, and right and left pulmonary artery remnants shall be recorded in centimeters (cm), as measured along the inferior (T/F) - Correct Answer-True 1 How long can skin be stored at refrigerated temp? a. no longer than 24 hrs b. no longer than 7 days 1 d. the time interval from asystole to subjecting vascular tissue to transport solution and wet ice temp at retrieval - Correct Answer-the time interval from asystole to subjecting vascular tissue to transport solution and wet ice temp at retrieval According to AATB standards a cardiovascular quality assurance program needs to ensure that the microbiologic growth media used in the processing of CV cultures can support the growth of all of the following types of organisms EXCEPT a.anaerob ic b. fungus c. acid fast bacillis d. aerobic - Correct Answer-acid fast bacillis Regarding heart valve, when must info such as temp, condition of transport device, and date/time of movement into storage at the processing facility be documented? a. immediately upon retrieval of the heart b. prior to transport of the heart to the processing facility c upon receipt of the heart at the processing facility d. immediately following cryopreservation of the valves - Correct Answer- Immediately following cryopreservation of the valves Name the 2 properties required for the solution used for rinsing and packaging the procured heart for valves a. isotonic , sterile b. hypertonic, cold c. hypotonic, sterile d. sterile, colloid - Correct Answer-Isotonic and sterile In which of the following is the length of procured vessel crucial for future clinical conduit utilization? a. superior vena cava b. pulmonary veins c. inferior vena cava d. pulmonary arteries - Correct Answer-Pulmonary arteries To prevent possible cellular, tissue and matrix, degeneration, CV tissue should be kept and between at all times during the dissection process a. aseptic and 0 deg C to 10 deg C 1 b. sterile and 0 deg C to 11 deg C c.moist and 1 deg C to 10 deg C d cryopreserved and 1 deg C to 10 deg C - Correct Answer-moist and 1 deg C to 10 deg C Which of the following is NOT required documentation concerning the heart transport/packaging solution? a. solution expiration date b. solution lot number 1 c. volume of solution in the package d. type of solution - Correct Answer-Volume of solution in the package According to AATB standards, the minimum recommended weight for heart valve donors is a. 6 lbs b. 5 lbs c. 7 lbs d. 4 lbs - Correct Answer-6 pounds If the donor has been maintained in a cold, refrigerated environment, the maximum warm ischemic time is a. 24 hrs b. 10 hrs c. 12 hrs d. 4 hrs - Correct Answer-24 hours The maximum allowable total ischemic time for a heart valve donor who has been maintained at room temp prior to heart recovery is a. 24hrs b. 12hrs c. 39hrs d. 48hrs - Correct Answer-39 hours Package inserts for cardiovascular tissue will contain all of the following EXCEPT a. donor heart warm ischemic time b. date of dissection or cryopreservation c. donor heart cold ischemic time d. donor heart total ischemic time - Correct Answer-Donor heart warm ischemic time When procuring a heart for valve use, it is important to maximize the best aortic conduit length by transecting the arch distal to the a. superior vena cava b. brachiocephalic artery c. left common carotid artery d. left subclavian artery - Correct Answer-Left subclavian artery All of the following criteria regarding the post retrieval packaging and 1 d. never - Correct Answer-2 weeks What year did the FDA issue the Interim Final Rule for Human Tissue? 1 a. 1993 b. 1997 c. 1995 d. 2001 - Correct Answer-1997 The minimal weight for a cardiovascular donor is generally a. 1 lb b. 6 lbs c. 10 lbs d. determined by the Medical Examiner - Correct Answer-6 pounds Split thickness skin is generally recovered at what thickness? a. .012 to .018 inches b. .018 to .035 inches c. determined by program SOP d. none of the above - Correct Answer-.012 to .018 inches Techniques for preserving musculoskeletal tissue include a. lyophilization b. dehydration c. freezing d. all of the above - Correct Answer-all of the above