Download Daily Quality Control of Reagents in Immunohematology | CLS 3311 and more Exams Chemistry in PDF only on Docsity! Daily Quality Control of Reagents PURPOSE: To provide guidelines for the CLS 3311 students to follow when they perform the daily quality control of testing reagents. The daily quality control demonstrates the reliability of our testing system, which includes reagents, methods, and test procedures, technologists and equipment. PRINCIPLE: The testing performed on these reagents are based on the principle of agglutination. Normal human red blood cells will agglutinate in the presence of antibody directed against antigens on those red blood cells. One method for quality control is the testing of known samples. If expected results are observed and documented, the tests are in control. If unexpected results are observed and documented, then the source of the problem must be determined prior to any patient testing. SCOPE: Students in CLS 2215 or CLS 3311 course at Weber State University EQUIPMENT: 12 x 75 mm test tube pipettes Serofuge (or Whisperfuge) Heat block 0.9% Saline Blood Bank record log REAGENTS: 1. Utilize one of the following Reagent Quality Control Kits: Gamma RQC Kit (Reagent Quality Control Kit) A. RQC A1B D-negative Red Cells B. RQC O D-positive Red Cells (0 R1r) C. RQC Antibody Reagent (diluted polyclonal and / or monoclonal antibodies including anti-A, anti-B as well as a mixture of IgG antibodies) Ortho Confidence System Kit A. Confidence Cell #1 (A1 B rr) B. Confidence Cell #2 (0 R1r) C. Confidence Antibody (diluted anti-A, anti-B, anti-D and anti-c) 2. ABO typing antisera 3. Rh typing antisera 4. Reverse ABO typing cells 5. Antibody Screen cells 6. Anti-Human Globulin (AHG) 7. Coombs Control -Check Cells 8. 0.9% sodium chloride 9. Patient with a previous known negative antibody screen. PROCEDURE: 1. Perform ABO-Rh red cell grouping and Rh Weak D (DU) typing using Gamma ROC A1B D-negative Cells or Ortho Confidence Cell #1. 2. Perform Rh typing using Gamma RQC O D-positive Cells or Ortho Confidence Cell #2. 3. Perform reverse grouping and antibody screen using Gamma RQC Antibody Reagent or Ortho Confidence Antibody. 4. Perform antibody screen using a known negative patient sample. 5. Record all reaction strengths (0 -4+) on the Blood Bank Record Log. 6. The Instructor or other qualified personal (check off students) will review results daily. 7. A reagent reactivity report is printed monthly for the Blood Bank Coordinator to review for any shifts or trends.
