Enzyme Action, Enzymes - Biology - Lecture Slides, Slides of Biology

Download Enzyme Action, Enzymes - Biology - Lecture Slides and more Slides Biology in PDF only on Docsity! Enzyme Action Learning objective: to describe different models for enzyme action and to investigate factors which control the rate of an enzyme reaction Docsity.com Lock and Key However certain substances can bind to the enzyme at sites other than the Active site and modify its activity (inhibitors/co-factors) Idea that the enzyme is flexible Docsity.com Enzyme reactions enzyme + substrate enzyme-substrate complex E +S ES Docsity.com Enzyme reactions enzyme + product enzyme-substrate complex E +P ES enzyme + substrate enzyme-substrate complex E +S ES Docsity.com Enzyme activity How fast an enzyme is working Rate of Reaction Docsity.com Four Variables Enzyme activity ZO __” ——“—- SN Docsity.com Enzyme activity Four Variables Temperature pH Enzyme Concentration Substrate Concentration Docsity.com R at e of R ea ct io n Temperature Docsity.com Effect of heat on enzyme activty If you heat the protein above its optimal temperature bonds break meaning the protein loses it secondary and tertiary structure Docsity.com Effect of heat on enzyme activty Denaturing the protein Docsity.com Effect of heat on enzyme activty Denaturing the protein ACTIVE SITE CHANGES SHAPE SO SUBSTRATE NO LONGER FITS Even if temperature lowered – enzyme can’t regain its correct shape Docsity.com R at e of R ea ct io n pH 1 3 4 2 5 6 7 8 9 Narrow pH optima Docsity.com R at e of R ea ct io n pH 1 3 4 2 5 6 7 8 9 Narrow pH optima WHY? Docsity.com R at e of R ea ct io n pH 1 3 4 2 5 6 7 8 9 Narrow pH optima Disrupt Ionic bonds - Structure Effect charged residues at active site Docsity.com R at e of R ea ct io n Substrate Concentration Docsity.com R at e of R ea ct io n Substrate Concentration Substrate Concentration Docsity.com R at e of R ea ct io n Substrate Concentration Substrate Concentration Active sites full- maximum turnover Docsity.com