Thermodynamics & Biochemistry: Constants, Equations, Chromatography, Inhibitors, Quizzes of Biochemistry

Download Thermodynamics & Biochemistry: Constants, Equations, Chromatography, Inhibitors and more Quizzes Biochemistry in PDF only on Docsity! TERM 1 Constants of thermodynamics DEFINITION 1 R: 8.315 x 10^-3 kJ/mol*deg T: 298K 1 kJ: 0.239 kcal TERM 2 Michaelis-Menten equation / Km / kcat DEFINITION 2 Vo = (Vmax*[S]) / ([S] + Km) Km = (k-1 + k2) / k1 kcat = k2 = Vmax / Et Catalytic efficiency = kcat / Km TERM 3 Two-Substrate reactions DEFINITION 3 Sequential kinetic mechanism: all substrate must bind to the enzyme before a product is formed Ping-pong (double-displacement) kinetic mechanism: one or more product is released before all substrates bind to enzyme TERM 4 Gel filtration chromatography DEFINITION 4 Separation by size Small molecules can enter beads of insoluble polymer and are eluted more slowly Larger molecules cannot enter the beads, and are eluted more quickly TERM 5 Gel electrophoresis DEFINITION 5 Sodium dodecyl sulfate (SDS) gives proteins uniformly negative charge, making the movement wholly contingent upon size of the proteins Small proteins move faster Larger proteins move slower TERM 6 Ion-Exchange chromatography DEFINITION 6 Cation-exchange chromatography (catches cations): positively charged protein binds to negatively charged bead, and negatively charged protein flows through first Anion-exchange chromatography (catches anions) TERM 7 De Novo pathway DEFINITION 7 Pyrimidines: rings are synthesized and added to PRPP to generate pyrimidine nucleotide / utilizes aspartate Purines: PRPP provides the foundation on which purine ring is synthesized / utilizes Asp, Gly, amine of Gln TERM 8 Pentasaccharide core DEFINITION 8 3 mannose and 2 N-acetylglucosamine TERM 9 Glycosylation patterns in RBC DEFINITION 9 O-antigen: none A-antigen: N-acetylgalactosamine B-antigen: galactose TERM 10 Rotenone DEFINITION 10 Related to Parkinson's disease Inhibits Complex I After NADH, everything remains oxidized