Fluorescence in-situ Hybridization Workflow Overview, Study notes of Medical Sciences

Download Fluorescence in-situ Hybridization Workflow Overview and more Study notes Medical Sciences in PDF only on Docsity! FLUORESGEAGEIN-SITE HYG EL DILATION zasuy 7 TALUORESCEIT IN-SITU NYWRIDIZAION” 7 MORE ROMUST becawsy it can detect microabnormalitits that can't ioe, detected by ROUTINE WARY OTYPING . | > done, after laryotyping if the result is oO derivative chromeso- m— this leadt ‘or encompasies further testing 10 produce: more gcwurate result - —v ADVANTRGE: can be, done on ees that arc in METAPHASE STAGE (ion) jor When the ecllo are in INTER- PHAGE Couc.- isn) PAUPHNGE | METAPHAGE igh — ish aq be. Ly chromosomes ceil oe early seen OS HAVEAPHAGE ( NUCLENR IG — nue ish Ly tanned oe dong by yust routine haryoryping —? COAL, SO Bttect o particular Vocus in our genome uring ci PADOE . “Ud it (5 Cposd, we can dead ~ Mf MMe WS AY present in the genome , the prove can a Mar can bind i comple | reat ; mand + HF) wind v0 \t YAPWHAG eOMpLEMENtary was pairing - the produt w anughio |~ ‘NE prove Can't qtracn 10 our target ONA if te in wer the vample ofA and te | GF ONA form) thav's why they need ve un- prone Widded 10 VEDME singiv- etranded UNA. be Uxpurdl the 10GUS of interest  See eee ee ee ALGORITHM OF The PROG £59 OF FISH © Fae oe FIXED seeamE] — > @ kan | (guraffin- embedded ) | ~ tic prt reatwent for fanolin—fixed = Wont marrow | |_| para fit , emlocdded tissue, ~ TaaWe biopsy (fresh | OF formalin-fixed - | | |9 OHARA na ParaLFin - crmbedded) |_| ~ dcoNA 1 SSDNA ty enpule ioclyy Of - FFEUGION |COF [fing needle | interest ‘ fae Camear, rowdy sme, [at bw We PROOE emp if _ ‘e otea of wytorpin preparation ) || 0 avouortiog a unlized = Bone MaNOW Frepning ary |- - Sug WA 1 be bingy Cured | wt det 414 ofied) ta 6 FMDHeATIO-OFTEERION rf | fe | tonto PROeT qy & MiPLE 6 Lau | | le oe | CUMIGAL 4 sNoRFOLOEIU oATA | + FLUORORSMRL > Moston that cath mit ight FLUORES || —> WE CoN detec He ight anitted ar a vignal or with a color FISH WOAK ELON - WE Sort wl collected fresh or fixed) panent DNA- ~ Pabienk OWA will We DENATAAED (via eating) to open up cn facilitate, the ataunment of a probe . ~ Wasi! — If the prove hay succesfully attach to it tanget 10, because wey are comnglomennary Wher) the Pot carl be Uttected Wa fluorescence, My UVEWIPY, =f EMG AGrgel WF Not here, the PObE Wal MO ane tO attach to Ihergtore it Will ot wayned off » NO yignal wi ue seer’ - whe hyorid if made Up Of Nhe FaMpIE Od prog, DNA ( packed WI fluor = Sometimes , MME Freagtty Of TG FigMal Camngy WE Jee IN the analy sig |