Genetic Engineering - International Baccalaureate Biology - Lecture Slides, Slides of Biology

Download Genetic Engineering - International Baccalaureate Biology - Lecture Slides and more Slides Biology in PDF only on Docsity! Genetic Engineering • Altering the genotype of an organism by: 1. adding new DNA to it, or 2. changing its DNA Docsity.com Recombinant DNA Technology • Basic technique: • 1. Cut two DNA molecules into fragments • 2. Join them in the desired combination Docsity.com Restriction Sites EcoRI: HindIII: GAATTC AAGCTT CTTAAG TTCGAA Docsity.com Restriction Enzymes • Cut DNA and produce RESTRICTION FRAGMENTS of different sizes Docsity.com • Cutting DNA with certain RE will result in “sticky ends” on the DNA molecule Docsity.com genome "1 Plasmid Vectors • Scientists insert DNA gene into vector • Vector is transferred into bacterial cell where it replicates Docsity.com Importance of E. coli • The “laboratory mouse” of bacteria • Used in genetic engineering Docsity.com Cloning Docsity.com Why do we clone genes? • Production of drugs • To give desired properties to crops • Produce transgenic organisms Docsity.com Transgenic organisms • Gene from Arctic char fish inserted into tomato = Frost-resistant tomato Docsity.com