Exam 4 for AGR 3303: Genetic Engineering and Molecular Biology, Exams of Genetics

Download Exam 4 for AGR 3303: Genetic Engineering and Molecular Biology and more Exams Genetics in PDF only on Docsity! AGR 3303 Name:_____________________ Exam 4 (45 points) Friday June 20, 2003 A) Match one of the following terms with each description. (10 points) A. lacZ N. RAPDs B. genetic drift O. plasmid C. apoptosis P. PCR D. cDNA Q. YACs E. BACs R. Agrobacterium F. northern blot S. Southern blot G. gene pool T. GFP H. totipotency U. restriction enzyme I. GUS V. migration J. determination W. organogenesis K. X-gal X. RFLP L. transgenic Y. somatic embryogenesis M. differentiation Z. ligase ------ 1. The process by which specific regions of small quantities of DNA can be amplified. ------ 2. A genetically engineered organism. ------ 3. The gene responsible for blue/white color selection in transformation of cloning vectors. ------ 4. Process by which DNA is transferred to a membrane following gel electrophoresis. ------ 5. Process by which cells achieve final form and fuction. ------ 6. Reporter gene that turns plant cells blue if expressed. ------ 7. Represents only the coding region of a gene. B) Answer true (T) or false (F) questions by circling the correct answer. (13 points) 11. Segmentation genes determine the identity of individual segments during development. T or F 12. Apoptosis is a highly regulated process. T or F 13. Hox genes are homeobox-containing genes present in mammals. T or F 14. RFLPs only detect nucleotide differences within restriction enzyme sites. T or F 15. Antibiotic resistance genes in plasmid cloning vectors are there to select for successful transformation. T or F 16. A genomic clone will not contain the promoter or introns. T or F 17. Generally, plant cells are better than animal cells at being totipotent. T or F 18. The green fluorescent protein (GFP) gene was isolated from a jellyfish. T or F 19. Proteins encoded by the myoblast-determining genes bind with MADS-box proteins to form heterodimers. T or F 20. Homeotic genes code for DNA binding proteins. T or F 21. Overexpression of the bcl-2 gene in humans can lead to cancer by preventing cell death. T or F 22. There are no assumptions made in the Hardy-Weinberg Law. T or F 23. In silica data mining means to use the computer to find interesting genes in databases. T or F 35. The eyeless gene controls the development of an eye in A. Drosophila only B. many species including Drosophila C. only in mutants D. only in wild-type individuals 36. These segmentation genes divide each segment into posterior and anterior regions A. pair-rule genes B. gap genes C. segment polarity genes D. maternal effect genes 37. The Antennapedia gene is an example of a A. mutant gene B. floral gene C. pair-rule gene D. homeotic gene 38. Floral homeotic genes are members of the A. protooncogene family B. MADs-box family C. E-box family D. C. elegans family 39. ESTs stand for A. extra sticky thymines B. expressed sequence tags C. enzyme site types D. excellent science tests 40. You have discovered a new autosomal recessive trait in a population of island weasels for black tongues (bb) that occurs at a frequency of 1 in 8,000. Assume that the weasel population under study, estimated at 150,000 animals, is in Hardy- Weinberg equilibrium. a. What are the values for p and q? (2points) b. What is the frequency of heterozygotes in the population? (2 points) 41. A sample of 5,631 individuals in a population was examined for the MN antigens and the results were: 1,245 MM 3,421 MN 965 NN What is the frequency of each allele in this population? (2 points)