Comparative Analysis of Mediator Complex Subunits in Various Species, Study notes of Molecular biology

Download Comparative Analysis of Mediator Complex Subunits in Various Species and more Study notes Molecular biology in PDF only on Docsity! Sample MSF file before modifying the title lines >gi|21357189|ref|NP_649569.1| Mediator complex subunit 27 CG1245-PA [Drosophila melanogaster](Note: this is the unknown) MDKLNSTLTAVKNLRSNVRLCFEHLADGTDGESGEESRNKFVNDFQERFAAINSQIREVEQLINGLPVPP TPYSLGNTAYLAQETSQDRQALYPQLVNSYKWMDKVHDHSFLAFNNLNQNTLRRSYNYCSQKRQRLPFSS FNNDPDHIDKLLSEINTPPHTSYRIFRPFGTNAVTIVTISNVMKAAIVFKGVLIEWVTVKGFDEPLEHDD LWAESRYEVFRKVQDHAHSAMLHFFSPTLPDLAVKSYITWLNSHVKLFLEPCKRCGKFVVNGLPPTWRDL RTLEPFHEDCRNC >gi|157116185|ref|XP_001652785.1| mediator complex, subunit, putative [Aedes aegypti] MNLEPINNALSHLRMLRSSVGQVFETLGNGVRAEHGEEGKEQKFLQELQELLNGVNSNLREFETCINDLT PPQAPFNLANTAYLSLETNLERQALYPHLVQSYKWHDKLHEYSTFASVLLQQNSLKRSYYTNTKRRRSLP SSHLATPQTVDNLIGSIHFPNMNLKIVRPFMTNAILHITIARVLRAAVILKGLLIEWVTVKGFDESLLDG VDEHWTVSRHQVFRKVQDHAHSAMLHFFSPTLPDLAIRSFITWFRSYLTLFADPCKKCGKHLHNTLPPTW RDLRTLEPYHEECKQ >gi|28558979|ref|NP_004260.2| mediator complex subunit 27 [Homo sapiens] MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNEL ERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQM GVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFID RTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAP CQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ >gi|17564368|ref|NP_505386.1| MeDiaTor family member (mdt-27) [Caenorhabditis elegans] MQSNQQPTTSANASGPSGARISSGNIPHSQSMPVLQSGNTANRPSTAGNPAAGRPILTLPSNTTEKHVIA LNQWAEVMKSKLEKMRQKSKKALDESMKEHSTMEDLSRAREALSEMGNMYYELNRAAALKCRYNAVFDKR PLYKAVESSIQDLRQKDESAYQLFYKLQEAMDERTVIEKNIIAVSETFRQNAVHAPRVSRLSVPPPLKFS KHVEDANKGVFLAMDAVMNKTRGDLTWRFKRISHPCFRNSKSLIEVHYCARRPNSEKEFVPCMKAVIVLK YGILEDMIIGGEDEDLYNQEQILHSKRKVYREFTKSAKEIILCSPVTKFTAPTNFAQCNNYIQSYVNCFS TKCYYCKKHLRQFMPPTVVTRESSPIICHKLCLMSQVP Note: In addition to your unknown cDNA (amino acid file), you need to include at least three (3) additional amino acid FASTA files. These should be from distantly related groups of organisms, so that we can see domains that have be conserved over long evolutionary distances. The conserved regions will correspond to functional domains.