Lab 4- equillibrium constants, Lab Reports of Chemistry

Download Lab 4- equillibrium constants and more Lab Reports Chemistry in PDF only on Docsity! Iinformati Mame: 250 mL Flask Volume: 220,000 mL Species (aq) Molarity : - He 1LO0885e-7 | cr ‘LOOBESe-7 ir 0.00163844 co a 000163844 350 mi, aT 00483616 20,000 ml, Temperature: 25.1190 pH: 7.00 6. Make calculations about binding of the bases ta form the base pair. The binding reaction equation is A+7T 3 AT The equilibrium constant for this reaction is referred to as the “binding constant” for A and T, and AGe for the reaction is the “free energy of binding”. Determine the equilibrium constant and the free energy of the binding reaction. And show the calculation work in Data Sheet. 7. Design an experiment to determine the equilibrium constant and the free energy of binding reaction of C and G ‘to form GG. Hint: repeal Step 1-6, except replace A with C, and T with G. Record the equilibrium conaentrations of C, G and GG in the Data Sheet, Table 2. Determine the equilibrium cometant and the free energy of lhe binding reaction babween C and G. And show the calculation work in Data Sheet. &. Design an experiment to determine the equilibrium constant and the free energy of binding reaction of & and C to form a mis-match pair AC, Hint: repeat Step 1-6, except replace C with T, Record the equilibrium ‘concentrations of A, C and AC in the Data Sheet, Table 2. Determine the equilibraim constant and the free energy of the binding reaction between A and, And shew the calculation work in Data Sheet, Data Sheet Table 1: AT binding. The chemical equation is_A + T > AT [Alea | Tao TAT] oo a Temperature in °C | Temperature in K (lo35A > 1u35H c= |Ygset ee | 95° q4¢ 7 ss: \$,067-7 K(binding of AT) = tt. = 1. 63 sa4e73 [legl Fle = \ AG® (binding of AT) =-RT in k= #3 M246) In (180577) = - A495 Ame R is the gas constant 8.31 J/imol.K; T is the temperature in K.  fel. [Sha fea)... Temperature in *C Temperetere la ).wosaseTy DMAIEN | ovate = <a Hibinding of CG)= ls = een AT ‘* AG® (binding of CG} = “AT in K = -§.31 (2%) inf Fre*) = - 2.243 e4: -34 34 4h, Rie the gas. congtant 231 Ano k: Tis thee beenpenptuire in K. eee ‘The oig-miching resection is A + ea Ac ee eae ee inowiass [ocmaiaes | ieamnse| 2s | as A he Hipinding ot acj= Mine oc Owines | 297 AG? (binding of Ac) = AT inks “82 13%) |n(a 57) = -2.7 TInt Fis the gas constant 6.31 JimoLH: T is the teenperature in K. Post Lab Assignments 1. Does your remlient K ang 40° make merce in tone of they structure of DMAT In ie DMA double helen, a iS paired with 6G, ond an A if paeree weet a T_ Bethy empiaae. es, The Hand AG” vais do make Sere ii tems CF DIN Structure == Swe Hoa, bounding eF OG 1S So hge” ele Ak wie, More = Spm ple complex avid Aer best pagar, Moan 1 1S more tergqe_hcally — Few ombie. This ahigng woh, a roo, Siabliby dad Peay SpeciPiily WT Hae DAI AR Silt 2. Calculate the equiibiurn corsiant af 75°C ter the iodiewing reachion bom the value of AG" given. Show your work. = joa" Og) 12a) 9 20R) = AG = -92u EE CI : = 1.00 wet) ie -1 Kee 4.7