LABORATORY 28 FEMALE REPRODUCTIVE SYSTEM, Study notes of Histology

Download LABORATORY 28 FEMALE REPRODUCTIVE SYSTEM and more Study notes Histology in PDF only on Docsity! LABORATORY 28 FEMALE REPRODUCTIVE SYSTEM - (second of three laboratories) UTERINE TUBE AND UTERUS OBJECTIVES: LIGHT MICROSCOPY: Recognize the different regions of the oviduct, the mucosa including epithelium and extent of muscle layer. Recognize the three layers of the uterus and characteristics of endometrium at each phase of the menstrual cycle including glands and stroma and the changes that occur in the cells of these structures. Contrast appearance of cervix with body of uterus. Recognize the mucosa, muscularis and adventitia of the vagina. ASSIGNMENT FOR TODAY'S LABORATORY GLASS SLIDES SL 133 Oviduct, fimbria and upper ampulla SL 134 Ampulla and broad ligament SL 135 Ampulla and broad ligament, five months pregnancy SL 139 Uterus and late proliferative SL 138 Uterus, early proliferative SL 140 Uterus, early secretory SL 141 Uterus, fully secretory SL 58 Uterus, menstrual SL 142 Uterine cervix, adult SL 144 Vagina SL 120 Vagina and urethra SL 71 Vagina and bladder HISTOLOGY IMAGE REVIEW - available on computers in HSL Chapter 17, Female Reproductive System, Uterus Frames: 1144-1175 SUPPLEMENTARY ELECTRON MICROGRAPHS Rhodin, J. A.G., An Atlas of Histology Female reproductive system - oviduct, uterus and vagina pp.409 - 414 (Copies of this text are on reserve in the HSL.) 28 - 1 UTERINE TUBE (OVIDUCT) The uterine tube is a three-layered structure, consisting of mucosa, muscularis and an outer layer that is for the most part serosa or peritoneum. The oviduct can be divided into the infundibulum from which the fimbria extends toward the ovary, the ampulla is the longest part of the tube and leads to the isthmus that is narrow and lies adjacent to the uterus. From the isthmus a short intramural region extends through the wall of the uterus. In the sections that we will study, the mucosa has elaborate thin folds that in the ampulla extend into the lumen. A. FIMBRIA AND UPPER AMPULLA, adult. SL 133 (scan) (J. 22-17, 22-18; W. 19.14) 1. Ampulla (upper portion). Identify the different layers of the oviduct, the mucosa, muscularis and serosa then examine the epithelium and identify the ciliated and secretory (peg) cells that are present. Also, determine whether diffuse lymphoid tissue is located in the lamina propria (med, high) (Two cell types are indicated by red and blue arrows). What structural and/or functional changes do these undergo with varying hormone influences? 2. Fimbria (red arrows) – The epithelium of the fimbria is similar to that of the ampulla. Observe the composition of the stroma and condition of the vasculature. B. AMPULLA AND BROAD LIGAMENT. SL 134 (scan) 1. Compare the different layers of oviduct in this slide with those observed in the previous slide. 2. Broad Ligament. (a) Connective tissue, blood vessels, lymphatics, scattered smooth muscle and serosa. (b) Are any vestigial tubules (blue arrow) present? What do they represent? C. AMPULLA AND BROAD LIGAMENT, SL 135 (scan). 5 months pregnancy 1. Compare epithelium to SL 133 above. UTERUS The body of the uterus has three major layers: endometrium (mucosa), myometrium (muscularis), and perimetrium (serosa). The endometrium shows the most apparent changes during the menstrual cycle. Therefore, study of the uterus involves an understanding the changes in the endometrium that characterize each stage of the cycle. A. UTERUS. Study a representative slide of the body of the uterus (SL 139) and observe the general characteristics of all layers. 1. Myometrium: It is evident that the myometrium is very thick; it is the thickest of the three layers. Careful inspection of the myometrium reveals that it is vaguely divided into several layers. Arteries and veins with thick muscular walls course through the muscularis and give rise to branches that enter other layers. 2. Endometrium: As you observe the endometrium define the type of epithelium that forms the uterine lining and determine the type of glands present. Be sure to look for different cell types that invade this region, especially in the late secretory phase of the cycle. Find arterioles and venules. Distinguish between the functional and basal zones (functional, red arrow; basal, blue arrow) of the endometrium and observe which region shows the most significant changes during the cycle. 3. Is any perimetrium on the section? 28 - 2