Laboratory Report on Bacterial Growth, Lab Reports of Biochemistry

Download Laboratory Report on Bacterial Growth and more Lab Reports Biochemistry in PDF only on Docsity! Formative Assessment 2: VLA2: Bacteria Growth Virtual Lab I. INTRODUCTION Microorganisms are everywhere - a largely unseen world of activities that helped to create the biosphere and that continue to support the life processes on earth. So, Welcome to the Microbial World. In fact, you are part of it. Of all the cells that make up the normal, healthy human body, more than 99 percent are the cells of microorganisms living on the skin or in the gut, etc. This normal resident microbial population includes potential pathogens as well as organisms that help to keep the potential pathogens in check II. OBJECTIVES After viewing the short video, reading the info sheet and virtual lab activity for this module, you will be able to: 1. explain the size of bacteria 2. classify bacteria based on the shape and arrangements 3. explain the factors affecting the growth of bacteria Specifically, This bacterial growth simulation allows students to work on experimental design, controlling variables and other aspects of the scientific method. III. PROCEDURE: 1. Using the set up from the link given http://bit.ly/3on9JjN, work on experimental design, controlling variables and other aspects of the scientific method by changing the setting for each of these factors. 2. Discuss the virtual set up you opted to tick. 3. Answer the guide questions in complete sentence(s). Bacteria Growth Virtual Lab Introduction and Background Today. you will design an experiment growing bacteria. You get to decide what you want to test: *Temperature *Antibacterial Substance A *Antibacterial Substance B *Experiment Duration In order to be successful, it will be important to apply the principles of good experimental design, such as controlling variables, running multiple trials, etc. Petri Dish Above, you can see several bacterial colonies that have grown on the dish. Each colony represents one bacterium that has survived and reproduced on the Petri dish. The size of the colony depends on how much the bacteria have reproduced in that colony. Introduction and Background Petri Dish There are several other variables that could affect your experimental results: * The amount and types of nutrients in the Petri dish agar. *The amount of bacteria initially added to the Petri dish. *The source and species of bacteria used. * Aseptic technique prevents contamination and involves wearing gloves and masks, proper inoculation techniques, sterilization of equipment, and not sneezing all over your Petri dish. ‘You will be given the option to control these (keep them constant between trials). A good experimental design is critical to your success! To learn more about growing bacteria, see the links in the description! eo borderfree classroom 5. In combining the set up you made on experimental design, controlling variablesand other aspects of the scientific method, what do you think are the necessary considerations for these factors? The following were the considerations we made the proper temperature setting ups to see when the bacterial growth will peak and then fade. The variables that were considered were the things that would help the bacteria grow as much as possible, which in this case were giving it nutrients and then eliminating some competition for the said nutrients, which is why nutrients in agar and antiseptic technique were ticked. For two days, the bacterial growth was allowed to multiply. Trying out different temperature, amount of substance, and duration of experiment, helped identify how microorganisms grow that would not turn it into other bacteria or mold. A temperature of 20 degrees Celsius, a substance of 20 and 5 and a duration of 2 days made possible for the bacteria to grow into a colony. Realization: Temperature, nutrients, pH level, moisture, and the presence of elements all have an impact on microorganism growth. Food is required by all microorganisms. The organisms' food sources vary, but they primarily extract carbon and nitrogen from proteins, fats, and carbohydrates. Such particles are sought after and absorbed by some microorganisms. In general, the higher the temperature, the easier it is for microorganisms to grow up to a point. Microbes grow faster at temperatures of 20, 25, and 30 degrees Celsius, according to Angela (n.d.). Temperature increases enzyme activity; however, if temperatures rise too high, enzyme activity decreases and the protein denatures. Lowering the temperature, on the other hand, reduces enzyme activity. Both extremely high and extremely low temperatures obstruct the enzyme processes that microorganisms rely on to survive. Microorganisms also prefer a specific pH level in the substance or environment in which they grow—that is, they prefer to be in an acidic environment. Microorganisms require free flow of water in order for their cells to exchange materials and perform metabolic processes. In addition to water, microorganisms usually require the presence of specific elements in the air—gases that they absorb in order to produce necessary nutrients. Nitrogen, like oxygen, is a necessary element. Reference(s): https://www.fdacs.gov/Consumer-Resources/Health-and-Safety/Food-Safety-FAQ/What- conditions-encourage-bacteria-to-grow https://bio.libretexts.org/Bookshelves/Microbiology/Book%3A_Microbiology_(Kaiser)/Unit_ 7%3A_Microbial_Genetics_and_Microbial_Metabolism/17%3A_Bacterial_Growth_and_En ergy_Production/17.1%3A_Bacterial_Growth#:~:text=Generation%20time%20is%20the% 20time,closer%20to%205%2D10%20hours. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941987/#:~:text=Aseptic%20technique%2 0refers%20to%20a,experiments%20that%20require%20growing%20cells. https://sciencing.com/three-conditions-ideal-bacteria-grow-9122.html