Enzyme Reaction Rate Experiment: Catalase and Hydrogen Peroxide, Lab Reports of Biology

Download Enzyme Reaction Rate Experiment: Catalase and Hydrogen Peroxide and more Lab Reports Biology in PDF only on Docsity! Bio 114 Enzyme Lab Instructions 1. *You must wear goggles for this lab! Your lab instructor will provide goggles for you to wear during this lab. 2. Add 3 ml of hydrogen peroxide (H2O2) to each of 2 test tubes. 3. Place tube #1 on ice and leave tube #2 at room temperature for 15-20 min. in order to allow the tubes of substrate to acclimate (get used) to their two respective temperatures. 4. After the 20 min. is up, add 10 drops of cold catalase solution to each tube, leaving them at their respective temperatures. 5. Let the reaction between the hydrogen peroxide and catalase occur in each tube (at 4 their respective temperatures) for exactly 3 min. 6. Stop both reactions by adding 3.5 ml of sulfuric acid (H2SO4) to each tube. *Note: You must add at least an equal volume of sulfuric acid (3.5+ ml; equal volume to the amount of hydrogen peroxide and catalase) or your titration produce a brown precipitate and inaccurate results, instead of the bright pink color that you should see. 7. Pour the contents of tube #1 into a small beaker. 8. Use the buret containing potassium permanganate (KmnO4) to titrate the amount of substrate (hydrogen peroxide) left: (1) Note the volume level of the liquid in the buret before you titrate. (2) Perform titration: Add the potassium permanganate solution from the buret into the flask until the liquid in the flask turns pink. Note: Don’t use more than 15 ml total to titrate. If after using 15 ml the solution in the beaker still doesn’t turn pink, then stop and just call it 15 ml. (3) Note the ending volume level of liquid in the buret. (4) Subtract the first buret volume reading from the second in order to find the amount used for titration. 9. Repeat steps 6. and 7. for tube #2. 10. Remember that in this titration you are measuring the how much substrate was used up in each reaction, which is an indirect measure of the actual rate of reaction. 11. Rinse all labware and leave on your table for the next lab.