DNA Replication & PCR: Denaturation, Renaturation, Synthesis & Applications, Quizzes of Agricultural engineering

Download DNA Replication & PCR: Denaturation, Renaturation, Synthesis & Applications and more Quizzes Agricultural engineering in PDF only on Docsity! TERM 1 how do you denature and renature dna DEFINITION 1 denatured with heat or alkaline conditionsrenatured by cooling or returning to neutral ph TERM 2 what synthesizes dna and in what direction DEFINITION 2 dna polymerase5-3' directionneeds a primer to initiate dna synthetsis TERM 3 how is dna replicated DEFINITION 3 semi conservativelyeach strand acts as a template to produce a new hybrid molecule TERM 4 what is pcr DEFINITION 4 polymerase chain reactiona technique for the selective amplification of a specific dna fragment TERM 5 what is the pcr cycle DEFINITION 5 repeating cycle ofdenaturation- heating of dnaannealing- hybridization of oligonucleotide primerextension- dna synthesis TERM 6 pcr process DEFINITION 6 dna templateoligonucleotide primersdeoxynucleotide triphosphatesa temp stable dna polymerase TERM 7 what is taq dna polymerase DEFINITION 7 thermal stable dna polymerase that was isolated from a bacterium thermus aquaticusgrow at 80cwas discovered by dr brockpermitted automation of pcr TERM 8 what are pcr primers DEFINITION 8 single stranded dna molecules20-25 nucleotidesnumber of nucleotides determined by size of genome4> 3x10^9need a pair of primers5' ends of primers mark the start and end of the pcr product or amplicon TERM 9 pcr applications DEFINITION 9 genetic screeningpaternity testingdisease diagnosisforensic analysistesting of crime scene evidenceidenficiation of disease organizationstracing the origin of a disease outbreak TERM 10 pcr limitations DEFINITION 10 pcr is a very sensitive method for detecting the presence of a specific dna sampletheoretically only a single dna molecule is needed for pcrbecause of the high sensitivity contamination and false positives are a real concern TERM 21 why aren't the two maps the same DEFINITION 21 frequency is not uniform across the genome TERM 22 what is the value of genetic maps DEFINITION 22 the location of unknown genes can be mapped by linkage analysis TERM 23 what is marker assisted selection DEFINITION 23 selection based on the absence or presence of dna markerspolymorphisms in genes themselvespolymorphic markers that are closely linked to loci of interest TERM 24 marker assisted selection works best for traits DEFINITION 24 with low heritabilitydifficult or expensive to measurecannot be measured until after selection has occurredsex limited TERM 25 blad DEFINITION 25 autosomal recessive diseasea common sire was found in pedigrees of infected animals28% of holsteins tested positive for blad carriers