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Neurophysiology Lab Exam: Ion Channel Analysis and Membrane Potentials - Prof. William J. , Exams of Animal Anatomy and Physiology

A lab exam focusing on neurophysiology, specifically ion channels and membrane potentials. Students are required to calculate missing data, apply the nernst equation, and analyze the effects of drugs on ion channels. Topics include action potentials, epsps, ipsps, and the role of acetylcholine and nicotine.

Typology: Exams

Pre 2010

Uploaded on 05/10/2008

koofers-user-miw
koofers-user-miw 🇺🇸

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Download Neurophysiology Lab Exam: Ion Channel Analysis and Membrane Potentials - Prof. William J. and more Exams Animal Anatomy and Physiology in PDF only on Docsity! £501 2993 W. J. Higgins NAME Round I Lab section # or day and time DIRECTIONS 1. PRINT your name on each page of the examination NOW!!!! 2. Please make certain that you have 5 pages. 3. Confine your answers to the spaces provided. You must use a pen if you wish the right to a regrade request. Examinations written in pencil will not be regraded. 4. Read the first in this year's series of deathless quotes, and then begin. "Somewhere I read the definition of a student as a person in the zealous and voluntary pursuit of knowledge." - Robert Day, 1993 SCOREBOARD Page Higgins Points Visitor 2. 36 3. 35 4. 85 5. 44 TOTAL = 200 points ______points Name #1. Dr. Harley Schmedlap, noted physiologist and hero to all 422 students, has discovered yet another interesting little beast while wandering through the Zoo- Psych Building. He has taken the following measurements from the beast's nerve cells and surrounding interstitial fluids. He has asked you, his trusty lab partner, to complete his study before sending the results to the Journal of Irreproducible Results. Ion G+ c- R+ A. rionlim 200 mM 1 10 4 Please supply the mis Tlonlout 20 mM 100 400 sing data by placing the reqi Eion Rel. Perm. 10 0 -61.5 mV 0.1 0.03 uested values (and signs where appropriate) in the blanks in the table above. B. Please utilize the Nernst equation to give an approximation of the Vm. Don't forget the sign! mV C. If during an action potential, the Relative Permeability of the nerve membrane to P++ increases to a value of 400, what value will the membrane potential approach? (Don't forget the sign!) ____________mV D. If only G+ and C" were permeable, would you observe a net movement or diffusion of C" across the membrane? Why or why not? E. List two changes in membrane permeability that would result in an EPSP at the dendrites of the nerves and then two that would yield an IPSP: EPSP: IPSR 1. 1. 2. 2. F. If Schmedlap observes that these axons are unmyelinated and small in diameter, what could you infer about the conduction velocity of these axons? Explain!
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