Molecular Fluorescence in Experimental Chemistry II - Lab 2 | CH 461, Lab Reports of Chemistry

Download Molecular Fluorescence in Experimental Chemistry II - Lab 2 | CH 461 and more Lab Reports Chemistry in PDF only on Docsity! CH 461 & CH 461H 14 F ‘08 October 10, 2008 Exp2B08.wpd Ingle, Pastorek, Westall Name Station # = Date DATA SHEET FOR EXPERIMENT 2B. Molecular Fluorescence. CH 461/461H VI: For each standard or sample solution, record Et and measure a blank and record Ebk. Promptly calculate Ef (eq. 7) and Ef' (see footnote a below). Table I. Fluorescence Calibration Data Conc. (:g/mL) Et (V) Ebk (V) Ef (V) SCM Gain (g) Ef' a (V) 100 1 75 1 25 1 10 1 2.5 2 1.0 5 0.25 20 0.1 100 syn. unknown 20 Z1 20 Z2 20 Z3 20 a Ef' is the normalized analyte fluorescence signal and calculated as Ef /g. By dividing the calculated fluorescence signal by the gain of the signal conditioning module (g), all fluorescence signals are normalized back to what they were at the output of the PMT module. You can assume that g is known to three significant figures. synthetic unk # PMT bias voltage = V Dark current voltage (Ed) = ______________ V CH 461 & CH 461H 15 F ‘08 October 10, 2008 Exp2B08.wpd Ingle, Pastorek, Westall VII: Detection Limit Data Include the computer printout of repetitive blank measurements and report the following: mean blank voltage = standard deviation in blank voltage = g = normalized standard deviation (referenced to PMT module output) = (the normalization is the same as used for the signals - see footnote a in the data table) Show calculation of normalized standard deviation: