Role of Non-Protein Nitrogen in Evaluating Kidney Function, Study notes of Physiology

Download Role of Non-Protein Nitrogen in Evaluating Kidney Function and more Study notes Physiology in PDF only on Docsity! Non-protein nitrogenous substances (NPN) often the first test conducted urinalysis isA simple, inexpensive screening test a routine if kidney problems are suspected. A small, randomly collected urine sample is examined physically for things like color, odor, appearance, and concentration (specific gravity); chemically, for substances such a protein, glucose, and pH (acidity/alkalinity); and microscopically for the presence of cellular elements (red blood cells [RBCs], white blood cells [WBCs], and epithelial cells), bacteria, crystals, and casts (structures formed by the deposit of protein, cells, and other substances in the kidneys’s tubules).  If results indicate a possibility of disease or impaired kidney function, one or more of the following additional tests is usually performed to pinpoint the cause and the level of decline in kidney function. 1-Glomerular function tests depend on examination of substances which depend on glomerular function for their elimination (creatinine, urea, and eGFR). The determination of non-protein nitrogenous substances (NPN) in the blood has traditionally been used to monitor renal function. The majority of these compounds arise from the catabolism of proteins and nucleic acids Non-protein nitrogenous substances (NPN): The most important NPN compounds used in evaluation of kidney functions are urea, creatinine, creatine uric acid, and ammonia. As clearance levels decrease, blood levels of creatinine, urea, and uric acid increase. 1-Blood Urea Nitrogen (BUN): Glomerular function test: It is formed in the liver from protein catabolism by releasing free amino groups (- NH2). Then it is transported to blood and then excreted in urine by kidneys . It represent about more than 75% of excreted NPN compounds. Urea is the major excretory product of protein metabolism. Its levels are affected by high protein intake, catabolic states, post-surgery and trauma, and gastro-intestinal hemorrhage, all of which cause increased urea production from protein. Physiology of Urea: Most of the urea in the glomerular filtrate is excreted in the urine, although some urea is reabsorbed by passive diffusion during passage of the filtrate through the renal tubules. Under conditions of normal flow and normal renal function, about 40% of the filtered urea is reabsorbed; when the flow rate is decreased, the amount passively reabsorbed increases. The amount reabsorbed depends on urine flow rate and extent of hydration. Small quantities of urea (≤10% of the total) are excreted through the gastrointestinal tract and skin. Small of urea portion is diffused to intestine to be cleaved by bacterial urease enzyme to CO2 and NH3. NH3 is partially lost in stool and partially reabsorbed to blood. The concentration of urea in the plasma is determined by renal function and perfusion, the protein content of the diet, and the rate of protein catabolism. The BUN test measures the amount of nitrogen contained in the urea. High BUN levels can indicate kidney dysfunction, but because BUN is also affected by protein intake and liver function, the test is usually done together with a blood creatinine, a more specific indicator of kidney function. The injection or ingestion of steroids produces a rise in BUN as do stressful situations that cause the adrenal gland to secrete additional cortisol. For these reasons, the measurement of serum creatinine is a better indicator of kidney status than is that of BUN although in many cases, they go up and down simultaneously. Urea nitrogen : serum 6-20 mg/dl , urine 24-h 12-20 g/day Pathophysiology of Urea: increased BUN is seen in the prerenal, renal, and post-renal factors. An elevated concentration of urea in the blood is called azotemia not uriceimia. Very high plasma urea concentration accompanied by renal failure is called uremia. This condition is eventually fatal if not treated by dialysis or transplantation. Prerenal azotemia is caused by reduced renal blood flow. It is increased the plasma urea levels without increased plasma creatinine. Less blood is delivered to the kidney; consequently, less urea is filtered. Causative factors include;  Decreased renal blood flow (renal ischemia) as in congestive heart failure (↓COP).  Shock and Significant decrease in blood volume. Renal azotemia: Decreased renal function causes an increase in plasma urea concentration as a result of compromised urea excretion.Intra- Renal causes of elevated urea include acute and chronic renal failure Post-renal azotemia can be due to obstruction of urine flow anywhere in the urinary tract by renal calculi, tumors of the bladder or prostate, or severe infection. It is But Creatinine clearance overestimates GFR because a small amount of creatinine is reabsorbed by the renal tubules and up to 10% of urine creatinine is secreted by the tubules. However, CrCl provides a reasonable approximation of GFR. Creatinine concentration decreases with age beginning in the fifth decade of life. Pathophysiology . Creatinine Renal causes of increased plasma creatinine include: Diseases with loss of nephrotic functions e.g. acute and chronic glomerulo-nephritis. Diseases with increased pressure on the tubular side of nephrones e.g. urinary tract obstruction due to prostatic enlargement. Plasma creatinine is a relatively insensitive marker and may not be measurably increased until renal function has deteriorated more than 50%. Low plasma creatinine: Creatinine production is determined by the size of creatine pool hence a smaller muscle mass leads to daily lower creatinine production. Physiologically pregnancy is accompanied with decreased plasma creatinine level. Also, females and children show low plasma creatinine levels when compared with adult men. Pathologically low plasma level of creatinine is found in wasting diseases, starvation, and in patients treated with corticosteroids due to their protein catabolic effect. Laboratories are making increasing use of estimated GFR derived from creatinine estimations, more especially because of the problems with measuring creatinine clearance((24 h) urine collection). Note : Creatine In muscle disease such as muscular dystrophy, poliomyelitis, hyperthyroidism, and trauma, both plasma creatine and urinary creatinine are often elevated. Plasma creatinine concentrations usually are normal in these patients. Measurement of creatine kinase is used typically for the diagnosis of muscle disease 3- Uric Acid: Uric acid is a metabolite of nucleic acids, purines, and nucleoproteins catabolism and is the end product of protein (purine) metabolism in man. Although it is filtered by the glomerulus and secreted by the distal tubules into the urine, most uric acid is reabsorbed in the proximal tubules and reused. Uric acid is relatively insoluble in plasma and, at high concentrations, can be deposited in the joints and tissue, causing painful inflammation. Physiology of Uric Acid: Purines, such as adenosine and guanine from the breakdown of ingested nucleic acids or from tissue destruction, are converted into uric acid, primarily in the liver. Uric acid is transported in the plasma from the liver to the kidney, where it is filtered by the glomerulus. Reabsorption of 98% to 100% of the uric acid from the glomerular filtrate occurs in the proximal tubules. Small amounts of uric acid are secreted by the distal tubules into the urine. Renal excretion accounts for about 70% of uric acid elimination; the remainder passes into the gastrointestinal tract and is degraded by bacterial enzymes. Nearly all of the uric acid in plasma is present as monosodium urate. At the pH of plasma (pH ˂7) urate is relatively insoluble; at concentrations greater than 6.8 mg/dL, the plasma is saturated. As a result, urate crystals may form and precipitate in the tissues. In acidic urine (pH˂5) uric acid is the predominant species and uric acid crystals may form Clinical Application of Uric Acid: Uric acid is measured to assess inherited disorders of purine metabolism, to confirm diagnosis and monitor treatment of gout, to assist in the diagnosis of renal calculi, to prevent uric acid nephropathy during chemotherapeutic treatment, and o to detect kidney dysfunction. Increased levels of uric acid have been observed in renal failure, chronic lead poisoning, polycythemia, some leukemias, and toxemia of pregnancy. Male 3.5-7.2 mg/dl Female 2.6-6 mg/dl Chronic renal disease causes increased uric acid concentration because filtration and secretion are impaired. However, uric acid is not useful as an indicator of renal function because many other factors affect its plasma concentration Hypouricemia is less common than hyperuricemia and is usually secondary to severe liver disease Ammonia: Ammonia is formed in the deamination of amino acids during protein metabolism. It is removed from the circulation and converted to urea in the liver. Free ammonia is toxic; however, ammonia is present in the plasma in low concentrations. Ammonia (NH3) is produced in the catabolism of amino acids and by bacterial metabolism in the lumen of the intestine. Some ammonia results from anaerobic metabolic reactions that occur in skeletal muscle during exercise. Ammonia is consumed by the parenchymal cells of the liver in the production of urea. At normal physiologic pH, most ammonia in the blood exists as ammonium ion (NH4+ ). Ammonia is excreted as ammonium ion by the kidney and acts to buffer urine. Clinical conditions in which blood ammonia concentration provides useful information are hepatic failure, Reye’s syndrome, and inherited deficiencies of urea cycle enzymes. measurement of urine ammonia can be used to confirm the ability of the kidneys to produce ammonia (the disease is preceded by a viral infection and the Reye’s syndrome :1ote N administration of aspirin ) . Ammonia is of use in the diagnosis of inherited deficiency of urea cycle :2ote N enzymes.