Download Notes on Advanced Clinical Immunohematology | CLS 3311 and more Study notes Chemistry in PDF only on Docsity! CLS 3311 Advanced Immunohematology Objectives for Rh blood group system lecture: The student will have the opportunity to: 1. Compare the theories of inheritance of the Rh system in respect to the investigators, terminology’s, genetic control and conformation to current theories. Describe the current proposed genetic theory of the Rh system. 2. Describe the characteristics of the first five Rh system antigens and their antibodies with regard to: enzyme treatment, dosage, complement binding, reaction temperature, pH, enhancement media, immunoglobulin class, placental passage, clinical significance, and number/location of antigen sites on cell. 3. Convert from the Fisher-Race to the Weiner nomenclature and vice versa. 4. Compare and contrast the three mechanisms explaining the ‘Weak D’ antigen. 5. Explain why knowing the D status is required in donor testing and not required in recipient testing. 6. Interpret Rh antisera typing results (D, C, c, E, e) in both Wiener and Fisher/Race nomenclature and using the phenotype frequencies indicate the most probable genotype (include any additional possible genotypes when requested). 7. Describe the reagents used for detection of the D antigen, including monoclonal blend, monoclonal/polyclonal blend, high protein and chemically modified indicating the purpose and contents of the control for each reagent. 8. Identify the frequency of the five major Rh antigens. List the frequencies of the five most common Rh haplotypes for White, Black and Asian populations. 9. Compare and contrast the following Rh antigens according to definition, frequency, antigenic reactivity, etc.: G (DC), f (ce), -D-, Ce, Cw, V and VS. 10. Analyze the possible causes of a positive Rh control and why this invalidates the Weak D test. 11. Examine the Rh-null phenotype identifying the several genetic explanations, antigen expression, peripheral blood findings, etc. 12. Explain Rh Immune Globulin according to its content, function and use. 13. List the first five most common Rh haplotypes, in order of frequency, using the Fisher-Race, Weiner, and Rosenfeld nomenclatures.
