Biology Lab Terms and Definitions: Media, Microscopes, and Microorganisms, Quizzes of Biology

Download Biology Lab Terms and Definitions: Media, Microscopes, and Microorganisms and more Quizzes Biology in PDF only on Docsity! TERM 1 energy source DEFINITION 1 phototrophs and chemotrophs TERM 2 carbon source and nitrogen source DEFINITION 2 heterotrophs (outside sourcesautotrophs (produce themselves) TERM 3 minerals DEFINITION 3 needed as cofactors for man cellular reactions TERM 4 water DEFINITION 4 needed to get nutrients into cell and maintain osmotic pressure TERM 5 liquids media DEFINITION 5 broth TERM 6 solids media DEFINITION 6 typically have 1.5% agar concentrationdeep agaragar slantsagar plates TERM 7 non-synthetic media DEFINITION 7 also known as complex mediacontain ingredients of unkown chemical compiundsprepared with extracts of meat, yeast or othersex. TGY (trypton, glucose, and yeast extract) TERM 8 synthetic media DEFINITION 8 also known as chemically defined mediaall ingredients are pure chemicals with known chemical formulasex. E. coli synthesis media TERM 9 selective media DEFINITION 9 ingredients that allow one organism to grow and inhibits anotherex. Mackonkey has bile salts which select for enteric bacteriaSodium Azide inhibits cytochrome TERM 10 differential media DEFINITION 10 contains ingredients which show differences between groups od bacteriaex. MacConkeys: pick indicates lactose fermentation TERM 21 enzyme production DEFINITION 21 bacteria produce different enzymes that can be detected beased on their substrate preference and end product prodcued TERM 22 suger fermentation DEFINITION 22 media type:glucoselactose mannitolsucrosepositive= turns from green to yellownote: any yellow inside the tube is positive reaction TERM 23 indole DEFINITION 23 media type: tryptone brothreagent needed: 5-8 drops of Kovac's reagent neededpositive: red ring formed on topnegative= no red ring TERM 24 hydrogen sulfide production DEFINITION 24 media type: SIMS deeppositive: black TERM 25 MR-VP DEFINITION 25 media type: MR-VPMR: add few drops od methyl red to cultturepositive: redVP: add 5-8 drops of a-napthol and KOH TERM 26 gelatin hydrolysis DEFINITION 26 media type: ntrient gelatinpositive: gelatin was hydrolyzednegative: gelatin hydrolysis did not occur TERM 27 simmon citrate DEFINITION 27 media type: simmon citratepositive: bluenegative: green TERM 28 nitrate reduction DEFINITION 28 media type: nitrate brothpositive: red after SA and DMN (nitrite)Nitrate after step 1 and zinc dust TERM 29 starch hydrolysis DEFINITION 29 media type: starch platemust add gram iodine to platepositive: starch was hydrolyzed by amylase; looking for clear zonenegative: starch was not degraded due to loack of amylase TERM 30 catalase DEFINITION 30 media type: TGY platemust add 3 drops of 3% H2O2positive: bacteria begins to bubble TERM 31 oxidase reduction DEFINITION 31 media type: TGY platemust ass 5-10 drops oxidase reagent2- 3 minute waitpositive: bacteria takes on a dark blue colornegative; after wait time bacteria remains light blue TERM 32 urease activity DEFINITION 32 media type: urea brothpositive: hot pinknegative: orange TERM 33 sterilization DEFINITION 33 elimination of all viable microbes TERM 34 disinfection DEFINITION 34 use of agents to inhibit growth of pathogenss TERM 35 methods of sterilization DEFINITION 35 heat: flame, autoclave, boiling, pasteurization (63C, 30 mins)radiation: UV light, DNA mutation by thymidinefiltration TERM 46 beta hemolysis DEFINITION 46 complete breakdown of RBC, clear zone TERM 47 alpha hemolysis DEFINITION 47 partial breakdown of RBC brown-green color TERM 48 deep agar DEFINITION 48 determines motility and O2 concentration TERM 49 agar slants DEFINITION 49 prepare stock cultures and study culture characteristics TERM 50 agar plates DEFINITION 50 study culture characteristics