Principles of Genetic Engineering, Genetics - Biology - Lecture Slides, Slides of Biology

Download Principles of Genetic Engineering, Genetics - Biology - Lecture Slides and more Slides Biology in PDF only on Docsity! Principles of genetic engineering Docsity.com What is genetic engineering • Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. • Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all. Docsity.com Step 1: Alternative method (using reverse transcriptase) • Reverse transcriptase • mRNA converted into cDNA • Complementary strand produced using DNA polymerase • Advantage – more mRNA in cell than DNA Docsity.com Step 2: Inserting gene into vector • Vector – molecule of DNA which is used to carry a foreign gene into a host cell Docsity.com Step 3: inserting vector into host ¢ See worksheet bacteria cells without plasmids} mest cells do Not take up plasmid sp‘ead thinly er to agar plate bacterial DNA alectroporatian teteevalna * 10 + 0 0 a few cells take 0 0 up plasmid and become transfonned plasmid vectors untransformed cells are killed by tetracycline transformed calls survive Docsity.com