Review Sheet for Principles of Chemistry Honor Lab II | CHM 218H, Study notes of Chemistry

Download Review Sheet for Principles of Chemistry Honor Lab II | CHM 218H and more Study notes Chemistry in PDF only on Docsity! EFT Summary Commands H1 spectra 0. Place sample in spinner to measured depth. Place in the nmr and turn the air off using the elbow switch; then on. You should hear a "purr" at this point. 0. Enter the PNMR program. 0. Select H1 parameters. <nu h1> Commands within <> are meant to be typed in the small command line towards bottom of screen. End the command with an "enter". Parameters should be ns=1; rg= 5 for neat samples; rg=30 for 2% samples and for 1-98 ethyl benzene standard.. 1. Acquire data. <zg> The computer will ask for a file name. Hit return to use the default name. The FID should be above base line and yellow. If red reduce rg. If there is no signal or very little; increase rg or run standard to find problem. 2. Enter NUTS program. Nut commands are just two letters in the main program with NO following enter. If you mistakenly enter a subprogram hit enter to get back to the main program. If the NUTS program is doing strange things; quit it and restart the NUTS application program. 3. Process the data. [Ctrl F2]. To use the default data file hit return when it asks for the data file name. 4. Trim the phase. [ap] 5. Integrate the spectrum [ai] 6. Plot the spectrum [pl] 7. If at any point, you have trouble run the 1-98 ethyl benzene standard to see if the problem is with the instrument or if it is with your sample. If it is the instrument problem contact JWL. Integration tricks for EFT spectra: If the integration scale is either too large or too small you can correct it by using the following procedure: step action explaination 1. use the command id puts you in the integration display menu 2. use the scroll bar on the left to adjust the scale 3. enter exits the integration display submenu If you want to put a numerical value on the integration: step action explaination 1. use the command id puts you in the integration display menu 2. put a cursor on one of the integrations. 3. use the command v This will bring up a window where you can put the value you want on the integration of this peak. Other peaks will be give relative to this. 4 enter exits the integration display submenu Phasing tricks for the EFT spectra. If the phasing of the spectrum is not good. You can manually phase the spectrum by: step action explaination 1. close the nuts window and restart a new one. clears any bad information out. 2. Do the basic processing the same way as usual- control F2 for proton spectra If the spectrum looks good at this stage do not phase. Just integrate and plot. 3. use the command PH This will put you in a manual phase subroutine. Hold down the left mouse and drag it left and right to do a first order correction. Hold the right mouse down and drag it left and right to make a second order correction.. 4 enter exits the manual phase submenu Clearing carry over information from your window. step action explaination 1. under the view unclick the appropriate items such as clipboard, peak labels.