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Terms & Concepts, Week 6
DNA siructureâfinction, continued from Week 5
complementary strands (A to T.G to C) See Fig. 9-3
DNAâs âinformationâ is contained in the sequence of N-bases {base triplet codesrecipe for protein)
semiconservalive replication (of DNA) - sce Fig. 9-4:
-helicase (âunzipsâ double helix)
-DNA polymerase (assembles new. complementary stand along each old strand}
-replication fork & bubble
mutation (change in a genc/DNA sequence)
RNA - single-stranded polymer of nucleotides
3 main types of RNA:
-ribosomal RNA (rRNA)
-messenger RNA (MRNA}
-transfer RNA (tRNA) -- see Fig. 9-11
gene segment of DNA with code for | proteinâpolypeptide chain
genes âworkâ by interacting with RNA Lo make proteins
Protein Synthesis (2 steps in prokaryotes, 3 steps in eukaryotes) - see Fig. 9-8
-transcription (NA > RNA) (always step 1)
-translation (RNA > protein) (step 2 in prok.: step 3 in eukaryotes)
-RNA splicing (step 2 in eukaryotes; makes MRNA)
franscription (see Fig. 9-10)
RNA polymerase - assembles RNA along one strand of DNA
codon (in mRNA) - base triplet coding for | amino acid â sec Fig. 9-9
stop (or nonsense) codon - can separate codes for different proteins along one MRNA molecule
RNA splicing - step 2 in eukaryoles (pre-mRNA > mRNA) -see Fig. 9-19
exons (spliced together)
introns (removed in splicing)
transfer RNA ((RNA) â sce Fig. 9-1] âshuttlesâ a specific amino acid to proper position in translation
anticodons (only in (RNA)
Translation (last step) â3 types of RNA interact Lo assemble protein: like an âassembly lineâ ribosome is
the âfactoryââsile: MRNA is the âconveyor beltâ with instructions: tRNA is âcartâ holding an amino acid
Details:
mRNA attaches to ribosome; I codon on mRNA attracts (RNA (with its amino acid) with
complementary anticodon; 2" codon on mRNA attracts RNA (& amino acid) with complementary
anticodon: peptide bond forms between amino acids; mRNA slides across the ribosome so that the [ codan
comes off the ribosome and the next (3) codon moves on and attracts a tRNA with a complementary
anticodon; 1â tRNA detatches from mRNA and from its amino acid and goes to pick up another amino
acid molecule: process continues, with each mRNA codon (specifying an amino acid) attracting aiRNA
anticodon, with the tRNA âtransferringâ Lhe amino acid to the growing polypeptide chain (protein);
when proicin is complete, il is released: the mRNA can attach to another ribosome and the process
can begin again, to make another molecule of the protein
gene expression (a gene is switched on so its protein is made)
transcriptional âswitchesâ (e.g., transcription factors)
translational âswitchesâ (e.g., microRNA)
ditferentiation -- an unspecialived cell becomes specialized due to differential gene expression
cloning â produces genetically identical organisms because it Involves mitosis & cytokinesis c.g... cuttings
tissue culture technique to âcloneâ cellsâplants artificially -- e.g, 32,000 yr.-old frozen plant cells!
asexual reproduction (new individuals, genetically identical)
sexual reproduction (new individuals. genetically distinctâdifferent)
meiosis division of nucleus in which the number of chromosomes ts cut ip half
syngamy'- fusion of 2 haptoid cells (gametes) to form a zygote (- fertilization)
life cycle diagram (meiosis & syngamv alternate)
diploid (2n}
haploid (nj