Genetics Terminology: Classical Methods, Reverse Genetics, and Genetic Elements, Quizzes of Cell Biology

Download Genetics Terminology: Classical Methods, Reverse Genetics, and Genetic Elements and more Quizzes Cell Biology in PDF only on Docsity! TERM 1 Classical Genetic Methods DEFINITION 1 Looking for mutations in phenotype TERM 2 Reverse genetics DEFINITION 2 using a database to find protein of interest TERM 3 Allele DEFINITION 3 Variance of a gene/different forms TERM 4 Genotype DEFINITION 4 Set of alleles under examination TERM 5 Phenotype DEFINITION 5 Physical outcome of genotype TERM 6 Wild Type DEFINITION 6 Most common allele; as it occurs in nature TERM 7 Recessive vs Dominant DEFINITION 7 Recessive --> loss of functionDominant --> gain of function TERM 8 Point Mutation DEFINITION 8 Changing one nucleotide:Silent, missense, nonsense TERM 9 Silent Mutation DEFINITION 9 Mutation does not result in change to Am. Acid sequence TERM 10 Missense DEFINITION 10 Mutation results in codon that codes for a different amino acid TERM 21 Transformation DEFINITION 21 Bacteria cells take up plasmid (only a small number will uptake) TERM 22 DNA Library DEFINITION 22 In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning. TERM 23 ECOR1 DEFINITION 23 EcoRI is an endonuclease enzyme isolated from strains of E.coli, and is part of the restriction modification system. TERM 24 Sticky End DEFINITION 24 A double-stranded DNA that only has one strand from the result of an endonuclease TERM 25 AMP1 DEFINITION 25 Ampicillin resistance gene TERM 26 DNA is negatively charged because it contains... DEFINITION 26 Phosphates TERM 27 Taq DEFINITION 27 Thermus aquaticus polymerase TERM 28 PCR DEFINITION 28 Polymerase Chain Reaction TERM 29 PCR Process... DEFINITION 29 Raise temperature to separate strands, only perfect complements will bind TERM 30 PCR Uses... DEFINITION 30 Amplify gene of interest by:1. Preparing probe for radiolabeling2. Reverse transcriptase (cDNA from mRNA) TERM 31 In situ hybridization DEFINITION 31 In situ hybridization is a type of hybridization that uses a labeled complementary DNA or RNA strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ) TERM 32 DNA microarray DEFINITION 32 Study expression of genes simultaneously (mRNA) TERM 33 Structural Motif DEFINITION 33 Combo of secondary and tertiary TERM 34 Leucine Zipper/Coiled Coil DEFINITION 34 Allophatic amino acid seriesUsed in Transcription factorsBinding in major groove to activate transcription TERM 35 EF hand/Helix-loop-helix DEFINITION 35 Binds Ca+When not bound to Ca+, is loose and open loop