Transcription Translation - Microbial Genetics - Lecture Notes, Study notes of Genetics

Download Transcription Translation - Microbial Genetics - Lecture Notes and more Study notes Genetics in PDF only on Docsity! Transcription, Translation, and Protein Folding I. RNA Structure Ribose vs Deoxyribose Uracil vs Thymine Properties: Reactivity, Stability, Secondary and Tertiary Structures II. Transcription RNA Polymerase and Chain Elongation 5'-3' Transcription Initiation: promoters Transcription Termination: factor independent and dependent termination Independent termination: stable hairpin and AAAAs Factor Dependent Termination: rho. Bind and Chase the polymerase III. Ribosomal RNA (rRNA) and Transfer RNA (tRNA) The bridge from transcription to translation IV. Protein Structure Amino acids join to make peptides and eventually proteins Primary, Secondary, Tertiary, Quaternary Structures V. Translation Ribosomes 50S (31 proteins + 23S rRNA + 5S rRNA) 30S (21 proteins + 16S rRNA) 70S the whole shabang tRNAs cognate aminoacyl-tRNA, Reading Frames The Genetic Code and Codons Redundant (The Wobble effect) Nonsense (stop) codons, Initiation codons, Codon Usage Translation Elongation EF-Tu, EF-G, P-site, A-site Translation Initiation formylmethionyl-tRNAfmet, initiation factors EF1 EF2 EF3 Removal of the Formyl Group and Methionine Translation Termination RF1 RF2 RF3, release? VI. Polycistronic RNA Polarity (translation influences transcription) and Coupling VII. Protein Folding Chaperones Cysteine and Disulfide isomerases Membrane sequences VIII. Antibiotics that Block Transcription and Translation docsity.com