Filogeografía y diversidad de X. montserratensis: un gasterópodo endémico en peligro, Diapositivas de Genética

¡Descarga Filogeografía y diversidad de X. montserratensis: un gasterópodo endémico en peligro y más Diapositivas en PDF de Genética solo en Docsity! Philogeography and diversity of the land snail Xerocrassa montserratensis • Land snail, < 1 cm shell length • Endemic species of Catalonia • Its distribution is restricted to Barcelona province • Listed as ENDANGERED in the Red List of threatened species by the International Union for Conservation of Nature. TG TG T TG TT TG TT T TG TT TT TG TT TT A TG TT TT AA TG TT TT AA T TG TT TT AA TT TG TT TT AA TT A TG TT TT AA TT AC TG TT TT AA TT AC A TG TT TT AA TT AC AT TG TT TT AA TT AC AT G TG TT TT AA TT AC AT G A TG TT TT AA TT AC AT G AC TG TT TT AA TT AC AT G AC T TG TT TT AA TT AC AT G AC TA TG TT TT AA TT AC AT G AC TA T DNA extraction Amplification (PCR) Gel electrophoresis Sequencer Sequencing reaction (PCR) Sequence reaction Sequencing reaction Only one primer (Forward or Reverse) Includes terminator dNTPs (ddNTPs) -Stop elongation -Had a fluorescent (colour) Adenine-Thymine-Cytosine-Guanine Range of coloured fragments Bands separated by size in a capillary Purification Purification a) Quantify genetic diversity per population. b) Construction of a haplotype network and a haplotype tree. c) Compute global and pairwise genetic distances. a) Quantifying genetic diversity m = sequence length (61) S = number of segregating sites (4) h = number of haplotypes (4) H = haplotype diversity (0.8) == (1-», pi?) k = average number of nucleotide differences between two sequences (1.933) 2 na —n(n-1) Si<j dij TT = nucleotide diversity (average number of nucleotide differences per site between two sequences) (0.0317) k m=>= m